Novel retroviral sequences are expressed in the epididymis and uterus of Syrian hamsters
Novel retroviral sequences are expressed in the epididymis and uterus of Syrian hamsters
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DOI：
10.1099/0022-1317-79-11-2687
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发表时间：
1998-11-01
影响因子：
3.8
通讯作者：
Norris, JS
中科院分区：
文献类型：
--
作者：
DeHaven, JE;Schwartz, DA;Norris, JS
雄激素和雌激素联合治疗雄性或雌性叙利亚仓鼠在生殖道平滑肌肉瘤的形成机制未知。我们研究了逆转录病毒基因表达可能在肿瘤发生中起作用的可能性，电子显微照片显示附睾和精液中有病毒样颗粒的证据。我们利用RT-PCR扩增了从附睾、睾丸、澄清精液和子宫分离的RNA中保守的逆转录病毒逆转录酶编码区，鉴定了表达的逆转录病毒序列，系统发育分析使我们将序列分为两个不同的组：(1)哺乳动物c型病毒，与Moloney小鼠白血病病毒、猫白血病病毒和长猿白血病病毒等相似；(2)混合ABCD组，如含有中国仓鼠和小鼠腹腔内a颗粒病毒序列、小鼠乳腺肿瘤病毒序列以及人类和猿猴逆转录病毒序列。Northern blot分析证实附睾和子宫中存在与哺乳动物c型病毒相关的全长逆转录病毒。然而，相对于未治疗的子宫，类固醇治疗并没有改变附睾或子宫肿瘤中逆转录病毒RNA的水平。总之，叙利亚仓鼠生殖组织被发现表达独特的逆转录病毒序列；然而，它们在激素致癌中的作用（如果有的话）仍未得到解决。
Combined androgen and oestrogen treatment of male or female Syrian hamsters results via an unknown mechanism in the formation of leiomyosarcomas in the reproductive tract. We have examined the possibility that retroviral gene expression may play a role in tumorigenesis, Evidence of virus-like particles in epididymis and seminal fluid is shown in electron micrographs. We identified expressed retroviral sequences by using RT-PCR to amplify a conserved retroviral reverse transcriptase coding region in RNA isolated from epididymis, testis, clarified seminal fluid and uterus, Phylogenetic analysis allowed us to classify the sequences into two distinct groups: (1) mammalian type-C viruses, having similarity to Moloney murine leukaemia virus, feline leukaemia virus and gibbon ape leukaemia virus amongst others; (2) a mixed ABCD group containing, for example, Chinese hamster and murine intracisternal A-particle virus sequences, mouse mammary tumour virus and human and simian retroviral sequences. The presence of putative full-length retrovirus related to mammalian type-C viruses in the epididymis and uterus was confirmed by Northern blot analysis. However, steroid treatment did not alter retroviral RNA levels in the epididymis or in a uterine tumour relative to untreated uterus, In summary, Syrian hamster reproductive tissues were found to express unique retroviral sequences; however, their role, if any, in hormonal carcinogenesis remains unresolved.