Correlation between the relative abundance of oral bacteria and Candida albicans in denture and dental plaques
Correlation between the relative abundance of oral bacteria and Candida albicans in denture and dental plaques
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DOI：
10.1016/j.job.2021.02.003
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发表时间：
2021-06-07
影响因子：
2.4
通讯作者：
Takebe, Jun
中科院分区：
文献类型：
--
作者：
Fujinami, Wakako;Nishikawa, Kiyoshi;Takebe, Jun
目的：机会性真菌白色念珠菌是义齿菌斑的组成部分，与义齿相关性口腔炎有关。在这种多物种生物膜中，白色念珠菌和细菌之间存在界间相互作用，这可能会影响斑块的微生物组成。本研究旨在调查假牙菌斑的细菌组成，以及这些细菌与白色念珠菌的相对丰度之间的相关性。方法：从 18 名假牙佩戴者（平均年龄 80.3 岁）中收集了 30 个假牙菌斑和 16 个牙菌斑样本。 DNA 提取后，使用针对细菌 V3-V4 高变区的 PCR 引物构建了元 16S rDNA 扩增子文库。通过高通量测序评估扩增子，然后进行细菌群体分析。通过实时 PCR 测定白色念珠菌 DNA 和总细菌 DNA 的浓度。通过Spearman等级相关分析各细菌属与白色念珠菌的相对丰度之间的相关性。结果：发现假牙中链球菌属、乳杆菌属、罗氏菌属和棒状杆菌属的丰度高于牙菌斑中的丰度。医疗相关肺炎的主要细菌也栖息在假牙表面。白色念珠菌与三种产酸菌呈正相关，与纤毛菌以及牙周炎和心内膜炎相关病原体呈负相关。结论：假牙可能是引起吸入性肺炎的病原体的重要储存库。与白色念珠菌呈负相关的细菌，例如细毛菌，可能有助于在抗真菌治疗中控制白色念珠菌的生长。 (C) 2021 年日本口腔生物学协会。由 Elsevier B.V. 出版
Objectives: The opportunistic fungus Candida albicans is a component of denture plaque and is associated with denture-related stomatitis. Inter-kingdom interactions between C. albicans and bacteria exist in such multi-species biofilms, which may affect the microbial composition of the plaque. This study was performed to investigate the bacterial composition of denture plaques, and the correlation between the relative abundance of these bacteria and C. albicans.Methods: Thirty denture plaque and 16 dental plaque samples were collected from 18 denture wearers (mean age, 80.3 years). After DNA extraction, a meta 16S rDNA amplicon library was constructed using PCR primers targeting the V3-V4 hypervariable region of bacteria. The amplicon was evaluated by high-throughput sequencing, followed by bacterial population analysis. The concentrations of both C. albicans DNA and total bacterial DNA were determined by real-time PCR. The correlation between the relative abundance of each bacterial genus and C. albicans was analyzed through Spearman's rank correlation.Results: The genera Streptococcus, Lactobacillus, Rothia, and Corynebacterium were found to be more abundant in dentures than in dental plaques. The predominant bacteria in healthcare-associated pneumonia also inhabited denture surfaces. C. albicans was positively correlated with three acidogenic bacteria and negatively correlated with Leptotrichia and pathogens associated with periodontitis and endocarditis.Conclusions: Dentures may be significant reservoirs of pathogens causing aspiration pneumonia. Bacteria showing negative correlation with C. albicans, such as Leptotrichia, may be useful for controlling the growth of C. albicans in antifungal therapies. (C) 2021 Japanese Association for Oral Biology. Published by Elsevier B.V.