Osteoclastic tartrate-resistant acid phosphatase (Acp 5): Its localization to dendritic cells and diverse murine tissues
Osteoclastic tartrate-resistant acid phosphatase (Acp 5): Its localization to dendritic cells and diverse murine tissues
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DOI：
10.1177/002215540004800207
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发表时间：
2000-02-01
影响因子：
3.2
通讯作者：
Cox, TM
中科院分区：
文献类型：
--
作者：
Hayman, AR;Bune, AJ;Cox, TM
抗酒石酸酸性磷酸酶(TRAP)是破骨细胞的组织化学标记物。它也是单组织细胞，特别是肺泡巨噬细胞的特征，并与各种病理条件有关，包括毛细胞白血病和艾滋病脑病。为了研究这种酶的生物学特性，我们研究了它在小鼠组织中的表达和活性。共聚焦荧光研究表明，TRAP定位于巨噬细胞的溶酶体室内。在成年小鼠中，该酶在骨、脾、肝、胸腺和结肠中活性较高，而在肺、胃、皮肤、脑和肾脏中活性较低。在睾丸、肌肉和心脏中检测到微量。原位杂交检测组织切片中TRAP mRNA的表达，组织化学染色或免疫组织化学方法检测蛋白表达。TRAP在许多组织中广泛表达，与主要来源于骨髓的细胞有关，包括破骨细胞/巨噬细胞系的细胞。TRAP mRNA和酶抗原在组织中的细胞分布与针对CD80(B7)抗原的抗体染色的细胞密切相关。因此，为了确定其在树突状细胞中的可能定位，分离的骨髓树突状细胞在培养中成熟。这些细胞共染色TRAP蛋白和CD80抗原。这些研究表明，TRAP是一种溶酶体酶，存在于不同的小鼠组织中，在树突状细胞以及破骨细胞和巨噬细胞中表达，正如先前显示的那样。
Tartrate-resistant acid phosphatase (TRAP) is a histochemical marker of the osteoclast. It is also characteristic of monohistiocytes, particularly alveolar macrophages, and is associated with diverse pathological conditions, including hairy cell leukemia and AIDS encephalopathy. To study the biology of this enzyme, we investigated its expression and activity in mouse tissues. Confocal fluorescence studies showed that TRAP is localized to the lysosomal compartment of macrophages. In adult mice, high activities of the enzyme were demonstrated in bone, spleen, liver, thymus, and colon, with lower amounts in lung, stomach, skin, brain, and kidney. Trace amounts were detected in testis, muscle, and heart. Expression of TRAP mRNA was investigated in tissue sections by in situ hybridization and protein expression was monitored by histochemical staining or immunohistochemically. TRAP is widely expressed in many tissues, where it is associated with cells principally originating from the bone marrow, including those of osteoclast/macrophage lineage. The cellular distribution of TRAP mRNA and enzyme antigen in the tissues corresponds closely to that of cells staining with an antibody directed to the CD80 (B7) antigen. Therefore, to confirm its putative localization in dendritic cells, isolated bone marrow dendritic cells were matured in culture. These co-stained strongly for TRAP protein and the CD80 antigen. These studies demonstrate that TRAP is a lysosomal enzyme that is found in diverse murine tissues, where it is expressed in dendritic cells as well as osteoclasts and macrophages, as expression previously shown.