Assay of erythrocyte membrane fatty acids. Effects of storage time at low temperature
Assay of erythrocyte membrane fatty acids. Effects of storage time at low temperature
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DOI：
10.1007/s005990070007
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发表时间：
2000-12-01
期刊：
影响因子：
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通讯作者：
Panico, S
中科院分区：
文献类型：
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作者：
Di Marino, L;Maffettene, A;Panico, S
研究饱和的单不饱和脂肪酸或多不饱和脂肪酸在血浆、循环细胞和组织中的稳定性，无论是酯化的还是未酯化的，对于验证低温储存在用于实验、观察、饮食或药理学研究的生物样品的使用是极其重要的。由于红细胞是很容易接触到的细胞，它们被用作较难接触到的组织的标志，特别是在大规模流行病学研究中。来自文献的数据表明，添加抗氧化剂和冻结红细胞在2-6个月至1年的时间内不会导致脂肪酸组成的任何变化。我们评估了从一名受试者的静脉血样本中分离出的红细胞中的脂肪酸浓度，这些红细胞用丁基羟基甲苯和新西兰Z保存，并在-80℃保存长达2年。6名受试者的静脉样本的红细胞在-20℃下保存6个月，不含丁基羟基甲苯，并在有空气的情况下用于比较。我们的数据表明，在N-2存在下，使用极低的温度(-80℃)和抗氧化剂(丁基羟基甲苯)时，长时间(2年)的储存对红细胞脂肪酸浓度没有显著影响。
The study of the stability of saturated mono-, or polyunsaturated fatty acids, both esterified and not esterified, in plasma, circulating cells, and tissues is extremely important to validate the use of biological samples stored at low temperature in "biological banks", which are used for experimental, observational, dietary, or pharmacological studies. Since red blood cells are easily accessible cells, they are used as a marker of less-accessible tissues, especially in large-scale epidemiological studies. Data from the literature suggest that the addition of an antioxidant and the freezing of red blood cells do not cause any variation in the fatty acid composition for a period of 2-6 months up to 1 year. We evaluated the fatty acid concentration in red blood cells isolated from venous blood samples of one subject, preserved with butylated hydroxytoluene and Nz and stored at -80 degreesC for up to 2 years. Erythrocytes of venous samples of six subjects stored at -20 degreesC for 6 months without butylated hydroxytoluene and in the presence of air were used for comparison purposes. Our data demonstrate that a long storage time (2 years) does not significantly influence the erythrocyte fatty acid concentration when using very low temperatures (-80 degreesC) and antioxidants (butylated hydroxytoluene) in the presence of N-2.