A REGULATED RNA-BINDING PROTEIN ALSO POSSESSES ACONITASE ACTIVITY
A REGULATED RNA-BINDING PROTEIN ALSO POSSESSES ACONITASE ACTIVITY
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DOI：
10.1073/pnas.88.22.10109
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发表时间：
1991-11-01
影响因子：
11.1
通讯作者：
KLAUSNER, RD
中科院分区：
文献类型：
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作者：
KAPTAIN, S;DOWNEY, WE;KLAUSNER, RD
铁响应元件（IRE）结合蛋白（IRE-BP）的克隆已被转染并在小鼠成纤维细胞中表达。IRE-BP基因产物以高亲和力和特异性结合IRE。氨基酸序列比对显示，IRE-BP与线粒体顺乌头酸酶有30%的同源性。线粒体顺乌头酸酶的18个活性位点残基与IRE-BP中的相同，表明IRE-BP可能具有顺乌头酸酶活性。纯化天然IRE-BP和免疫亲和纯化转染和表达的IRE-BP后，我们证明纯化的IRE-BP具有顺乌头酸酶活性。
A clone for the iron-responsive element (IRE)-binding protein (IRE-BP) has been transfected and expressed in mouse fibroblasts. The IRE-BP gene product binds IREs with high affinity and specificity. Amino acid alignments reveal that the IRE-BP is 30% identical to mitochondrial aconitase. The 18 active site residues of mitochondrial aconitase are identical to those in the IRE-BP, suggesting that the IRE-BP may possess aconitase activity. After purification of native IRE-BP and immunoaffinity purification of transfected and expressed IRE-BP, we demonstrate that the purified IRE-BP has aconitase activity.