Zebrafish hairy/enhancer of split protein links FGF signaling to cyclic gene expression in the periodic segmentation of somites
Zebrafish hairy/enhancer of split protein links FGF signaling to cyclic gene expression in the periodic segmentation of somites
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DOI：
10.1101/gad.1291205
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发表时间：
2005-05-15
影响因子：
10.5
通讯作者：
Takada, S
中科院分区：
文献类型：
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作者：
Kawamura, A;Koshida, S;Takada, S
Notch 和成纤维细胞生长因子 (FGF) 信号通路与脊椎动物体节适当周期性的建立有关。在这里，我们展示了斑马鱼中与 Hes6 相关的毛状/分裂相关基因增强子 her13.2 将 FGF 信号传导与 Notch 调节的振荡机制联系起来的证据。 her13.2 的表达由 FGF 浸泡的珠子诱导，并由 FGF 信号抑制剂降低。 her13.2 是Notch 调节基因her1 和her7 的周期性抑制以及正确体节分割所必需的。此外，Her13.2 增强了与 Her1 蛋白相关的 her1 的自身抑制。因此，FGF 信号似乎通过为 Her1 提供结合伴侣 Her13.2 来维持振荡机制。
Notch and fibroblast growth factor (FGF) signaling pathways have been implicated in the establishment of proper periodicity of vertebrate somites. Here, we show evidence that a Hes6-related hairy/Enhancer of split-related gene, her13.2, links FGF signaling to the Notch-regulated oscillation machinery in zebrafish. Expression of her13.2 is induced by FGF-soaked beads and decreased by an FGF signaling inhibitor. her13.2 is required for periodic repression of the Notch-regulated genes her1 and her7, and for proper somite segmentation. Furthermore, Her13.2 augments autorepression of her1 in association with Her1 protein. Therefore, FGF signaling appears to maintain the oscillation machinery by supplying a binding partner, Her13.2, for Her1.