PARASITIZATION BY Macrocentrus cingulum (HYMENOPTERA: BRACONIDAE) INFLUENCES EXPRESSION OF PROPHENOLOXIDASE IN ASIAN CORN BORER Ostrinia furnacalis
PARASITIZATION BY Macrocentrus cingulum (HYMENOPTERA: BRACONIDAE) INFLUENCES EXPRESSION OF PROPHENOLOXIDASE IN ASIAN CORN BORER Ostrinia furnacalis
复制标题
Macrocentrus cingulum（膜翅目：茧蜂科）的寄生影响亚洲玉米螟玉米螟中酚氧化酶原的表达
DOI：
10.1002/arch.20425
复制
发表时间：
2011-07-01
影响因子：
2.2
通讯作者：
Huang, Yongping
中科院分区：
文献类型：
--
作者：
Feng, Congjing;Huang, Jianhua;Huang, Yongping
从亚洲玉米螟Ostriniapericacalis中克隆了一个多酚氧化酶原（prophenoloxidase，PPO）cDNA（OfPPO）。序列分析表明，该基因全长2,686 bp，含有一个2,079 bp的开放阅读框（ORF）、一个73 bp的5 '端非翻译区和一个534 bp的3'端非翻译区，其中3 '端非翻译区含有poly（A）信号。ORF编码一个693个氨基酸的多肽，含有两个不同的铜结合区，一个合理的硫酯位点，两个蛋白水解激活位点，和一个保守的C-末端区域，但缺乏信号肽序列。在寄生后4 h（pp），带茧茧蜂抑制血浆、血细胞、脂肪体和中肠中OfPPO转录物的表达。原位杂交分析表明，血细胞，特别是类卵细胞，与OfPPO基因的DNA探针杂交强烈。在被寄生幼虫的表皮上皮或脂肪体中未检测到信号。胶体金颗粒用于通过免疫电子显微镜观察PPO。时间进程研究表明，在4，6，8，12，和1天PP的幼虫体壁和中肠中的标记的OfPPO的减少寄生的M。扣带我们从OfPPO基因表达和PO酶活性的时程研究中推断，在珠被中的OfPPO是从血细胞中释放的，并且OfPPO表达在转录、翻译和翻译后水平上受到寄生挑战的影响。(C)2011 Wiley Periodicals，Inc.
A prophenoloxidase (PPO) cDNA (OfPPO) was cloned from the Asian corn borer Ostrinia furnacalis. Sequence analysis revealed a full length transcript of the OfPPO cDNA with 2,686 bp, containing a 2,079 bp open reading frame (ORF), a 73-bp 5'-untranslated region, and a 534-bp 3'-untranslated region with a poly(A) signal. The ORF encodes a 693-amino acid polypeptide, containing two distinct copper-binding regions, a plausible thiol ester site, two proteolytic activation sites, and a conserved C-terminal region, but lacks a signal peptide sequence. Expression of the OfPPO transcript in the plasma, hemocytes, fat body and midgut was inhibited by Macrocentrus cingulum at 4 h post-parasitization (pp). In situ hybridization analysis showed that the hemocytes, especially the oenocytoids, hybridized strongly with the DNA probes of the OfPPO gene. No signal was detected in the cuticular epithelium or fat body of the parasitized larvae. Colloidal gold particles were used to visualize the PPO by immunoelectron microscopy. The time course study revealed a decrease in the labeling of the OfPPO at 4, 6, 8, 12, and 1 day pp in the larval integument and midgut parasitized by M. cingulum. We infer from time course studies of OfPPO gene expression and PO enzymatic activity that OfPPO in the integument is released from hemocytes and that the OfPPO expression was influenced at the transcriptional, translational, and then the post-translational level by parasitization challenge. (C) 2011 Wiley Periodicals, Inc.