Low-pH-induced fusion of Vero cells infected with Junin virus
Low-pH-induced fusion of Vero cells infected with Junin virus
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DOI：
10.1007/bf01718832
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发表时间：
1996-01-01
影响因子：
2.7
通讯作者：
Mersich, SE
中科院分区：
文献类型：
--
作者：
Castilla, V;Mersich, SE
用Junin病毒(JV)感染的Vero细胞研究病毒诱导细胞-细胞融合的能力。JV引起的多核细胞形成与pH和温度有关。BHK-21细胞的融合活性降低。不同毒株的融合活性表现出相似的程度和pH依赖性。通过免疫沉淀法检测抗主要病毒糖蛋白（GP38）的中和抗体抑制合胞体的产生和GP38对酸处理的构象变化。
Junin virus (JV) infected Vero cells were used to investigate virus capacity to induce cell-cell fusion. Polykaryocyte formation due to JV was found to be pH and temperature-dependent. A reduced fusion activity was detected on BHK-21 cells. Different JV-strains exhibited a similar extent and pH dependence of their fusion activity. Neutralizing antibodies against the main viral glycoprotein (GP38) inhibited syncytium production and GP38 conformational changes in response to acid treatment were detected by an immunoprecipitation assay.