A porcine model for fixed drug eruptions in humans: the case of antipyrine in the Yucatan micropig.
A porcine model for fixed drug eruptions in humans: the case of antipyrine in the Yucatan micropig.
复制标题
人类固定药疹的猪模型：尤卡坦微型猪的安替比林案例。
DOI：
10.1002/jat.966
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发表时间：
2006
期刊：
影响因子：
--
通讯作者：
Mayersohn,Michael
中科院分区：
文献类型：
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作者：
Pak,Yvonne;Stollberg-Zagar,Kathleen;Mayersohn,Michael
到目前为止，还没有可接受的动物模型来研究人类固定性药疹（FDE）。我们在这里简要报告的意见表明，尤卡坦小型猪可能是一个有用的动物模型，用于此目的。在安替比林吸收和处置的研究过程中，我们观察到静脉注射1 g剂量后FDE的发展。我们的观察结果是一致的，在几个调查报告的人服用单剂量的安替比林。为了证实这些结果，对未处理的微型猪进行了攻毒。对雄性未去势尤卡坦小型猪（27.2 kg）静脉注射1 g剂量的安替比林。30天后，用相同静脉内剂量的安替比林（1 g）再次激发该猪。采集血液样本以检查免疫学终点。在最初的挑战，流体斑块（约。1~1.5cm）。给药后6 h。再激发后，在最初形成水疱的相同部位观察到粉红色发炎斑块;但是，未重新形成水疱。攻毒后8 h观察到中性粒细胞、淋巴细胞和嗜酸性粒细胞水平较基线的变化。微型猪似乎没有受到FDE的影响。这些观察结果表明，尤卡坦微型猪，或一般的猪，可能是一个有用的动物模型，用于检测药物，可能会导致FDE在人类。版权所有© 2005年约翰威利父子有限公司。
To date, there is no acceptable animal model to investigate fixed drug eruptions (FDEs) in humans. We briefly report here observations suggesting that the Yucatan micropig may be a useful animal model for that purpose. During an investigation of antipyrine absorption and disposition, we observed the development of FDEs after intravenous administration of a 1 g dose. Our observations were consistent with those reported in several investigations of humans taking a single dose of antipyrine. To confirm these results, a naïve micropig was challenged. A male uncastrated Yucatan micropig (27.2 kg) was given a 1 g dose of antipyrine intravenously. After 30 days, this pig was rechallenged with the same intravenous dose of antipyrine (1 g). Blood samples were obtained to examine immunological endpoints. During the initial challenge, a fluid plaque (ca. 1–1.5 cm) appeared on the left hip of the pig ca. 6 h after dosing. After the rechallenge, inflamed pink patches were observed at the same sites where the blisters formed initially; however, no blisters re‐formed. Changes of neutrophil, lymphocyte and eosinophil levels from baseline were noted 8 h after challenge. The micropig did not seem otherwise affected by the FDEs. These observations suggest that the Yucatan micropig, or swine in general, may be a useful animal model for detecting drugs that may cause FDEs in humans. Copyright © 2005 John Wiley & Sons, Ltd.