Analysis of the Cell Surface Expression of Cytokine Receptors Using the Surface Protein Biotinylation Method
Analysis of the Cell Surface Expression of Cytokine Receptors Using the Surface Protein Biotinylation Method
复制标题
DOI：
10.1007/978-1-4939-0928-5_16
复制
发表时间：
2014-01-01
期刊：
影响因子：
--
通讯作者：
Yu, Yong
中科院分区：
文献类型：
--
作者：
Pavel, Mahmud Arif;Lam, Clarissa;Yu, Yong
细胞因子是调节对感染和炎症的免疫反应的多效性低分子量蛋白质。它们通过与细胞质膜上的细胞因子受体结合来刺激免疫应答。因此，了解细胞表面特定细胞因子受体的表达水平对于理解细胞因子的功能和调节至关重要。细胞表面生物素化是研究细胞膜上细胞因子受体的技术之一。生物素化的表面蛋白可以通过生物素和链霉亲和素之间的强相互作用快速纯化。在这里，我们描述了表面生物素化和纯化生物素化的细胞因子受体的程序，以进一步下游分析。
Cytokines are pleiotropic, low-molecular-weight proteins that regulate the immune responses to infection and inflammation. They stimulate the immune responses by binding to cytokine receptors on the cell plasma membrane. Thus, knowledge of the expression level of particular cytokine receptors on cell surface is crucial for understanding the cytokine function and regulation. One of the techniques to explore the membrane embedded cytokine receptors is cell surface biotinylation. Biotinylated surface proteins can be rapidly purified through the strong interaction between biotin and streptavidin. Here, we describe the procedure for surface biotinylation and purification of biotinylated cytokine receptors for further downstream analysis.