Baicalin attenuates endometritis in a rabbit model induced by infection with Escherichia coli and Staphylococcus aureus via NF-κB and JNK signaling pathways
Baicalin attenuates endometritis in a rabbit model induced by infection with Escherichia coli and Staphylococcus aureus via NF-κB and JNK signaling pathways
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黄芩苷通过 NF-κ B 和 JNK 信号通路减轻大肠杆菌和金黄色葡萄球菌感染引起的兔模型子宫内膜炎
DOI：
10.1016/j.domaniend.2020.106508
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发表时间：
2021-01-01
影响因子：
2.1
通讯作者：
Li, J.
中科院分区：
文献类型：
--
作者：
Miao, Y.;Ishfaq, M.;Li, J.
在本研究中，开发了兔子宫内膜炎模型来研究牛子宫内膜炎。此外，还研究了黄芩苷（一种黄酮类化合物）对子宫内膜炎的保护作用。临床症状、白细胞分类计数、子宫分泌物涂片镜检及化验、尿液检测、尸检体征均显示兔子宫有异常变化和炎症反应。组织病理学结果显示细胞间隙可见炎症渗出物和血斑，证实兔子宫内膜炎模型已成功建立。最重要的是，黄芩苷治疗部分减轻了这些炎症症状。数据显示，黄芩苷给药后，体温升高、白细胞、脓液和上皮细胞脱落得到缓解，且呈剂量依赖性。黄芩苷治疗可部分减弱炎症细胞浸润、充血、出血和上皮细胞脱落等组织病理学组织变化。此外，LPS处理后RAW264.7细胞中炎症相关基因（iNOS、IL-1β、TNF-α、IL-10、IL-4和IL-6）的mRNA表达显着改变。此外，LPS 处理后 JNK、p65 和 I kappa B α 的磷酸化蛋白表达显着降低。有趣的是，黄芩苷预处理逆转了炎症相关基因 mRNA 表达的变化，并显着降低了 JNK、p65 和 I kappa B α 的磷酸化。总之，我们的结果表明黄芩苷对兔细菌性子宫内膜炎具有保护作用，其涉及抑制 NF-κ B 和 JNK 信号通路以及促炎细胞因子。 (C) 2020 Elsevier Inc. 保留所有权利。
In this study, a rabbit endometritis model was developed to study cow endometritis. In addition, the protective effects of baicalin (a flavonoid) against endometritis were investigated. Clinical symptoms, differential leukocyte counting, uterine secretion smear microscopy and chemical examination, urine testing, and signs of necropsy showed abnormal changes and inflammatory responses in the uterus of rabbits. Histopathological results revealed visible inflammatory exudates and blood spots between intercellular spaces which confirmed that the rabbit endometritis model was successfully developed. Most importantly, these inflammatory signs were partially attenuated with baicalin treatment. The data revealed that the increased body temperature and leukocyte cells, pus, and the detachment of epithelial cells were alleviated with baicalin administration in a dose-dependent manner. Histopathological tissue changes such as inflammatory cells infiltrates, hyperemia, hemorrhages, and shedding of epithelial cells were partially attenuated with baicalin treatment. In addition, the mRNA expression of inflammation-related genes (iNOS, IL-1 beta, TNF-alpha, IL-10, IL-4, and IL-6) was significantly altered in RAW264.7 cells after LPS treatment. Further, the phosphorylated protein expression of JNK, p65, and I kappa B alpha were significantly reduced with LPS treatment. Intriguingly, baicalin pretreatment reversed the alteration in mRNA expression of inflammation-related genes and significantly reduced the phosphorylation of JNK, p65, and I kappa B alpha. In summary, our results suggest that baicalin has protective effects on bacterialinduced endometritis in rabbits that involve the suppression of NF-kappa B and JNK signaling pathways and pro-inflammatory cytokines. (C) 2020 Elsevier Inc. All rights reserved.