Multiphasic analysis of xanthomonads causing bacterial spot disease on tomato and pepper in the Caribbean and Central America: Evidence for common lineages within and between countries
Multiphasic analysis of xanthomonads causing bacterial spot disease on tomato and pepper in the Caribbean and Central America: Evidence for common lineages within and between countries
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DOI：
10.1094/phyto.1999.89.4.328
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发表时间：
1999-04-01
期刊：
影响因子：
3.2
通讯作者：
Jackson, LE
中科院分区：
文献类型：
--
作者：
Bouzar, H;Jones, JB;Jackson, LE
从4个加勒比海和中美洲国家的32个不同的领域的番茄或辣椒植物分离的433株黄单胞菌菌株进行了筛选，以水解淀粉和聚果胶钠的能力和对铜和链霉素的抗性。其中，95个代表性菌株进一步表征各种phnetic测试，63这些菌株进行了基因组指纹图谱分析。绝大多数菌株（>90%）对铜具有耐受性。然而，对链霉素的敏感性有更多的变异性。瓜德罗普的所有菌株和巴巴多斯93%的菌株对链霉素敏感。绝大多数菌株为典型的野油菜黄单胞菌致病变种。vesicatoria group A strains.然而，在巴巴多斯，鉴定了一组独特的菌株，其血清学上类似于A组菌株，但具有淀粉分解性。这些菌株被命名为Al。野油菜致病变种发现中美洲的水疱病B群菌株仅限于哥斯达黎加的两个田地和危地马拉的一个田地。在加勒比地区未发现B组菌株，而在美国中部和南美洲则很常见。本研究中未发现T3菌株，尽管最近此类菌株在佛罗里达和墨西哥有所增加。来自哥斯达黎加的X. gardneri群。除了在物种/致病变种水平外，在病原体的表型和REP-聚合酶链反应（REP-PCR）基因组指纹图谱之间发现很少的联系;发现显示几乎相同指纹图谱的菌株对应于不同的种族，反之亦然。rep-PCR基因组指纹分析表明，某些谱系可能在特定地区或特定国家进化或占主导地位。
Four hundred thirty-three xanthomonad strains isolated fi om tomato or pepper plants from 32 different fields in four Caribbean and Central American countries were screened for the ability to hydrolyze starch and sodium polypectate and for resistance to copper and streptomycin. Of these, 95 representative strains were further characterized by various phnetic tests, and 63 of these strains were then analyzed by genomic fingerprinting. Most of the strains (>90%) were tolerant to copper. However, there was much more variability in sensitivity to streptomycin. All strains in Guadeloupe and 93% of the strains in Barbados were sensitive to streptomycin. The majority of strains were typical Xanthomonas campestris pv. vesicatoria group A strains. in Barbados, however, a unique group of strains was identified that was serologically similar to group A strains but was amylolytic. These strains were designated Al. The occurrence of X. campestris pv. vesicatoria group B strains in Central America was found to be limited to two fields in Costa Pica and one in Guatemala. No group B strains were identified in the Caribbean, in contrast to common occurrence in the central United States and in South America. T3 strains were not found in this study, despite the recent increase of such strains in Florida and Mexico. Unique strains from Costa Rica belonging to the X. gardneri group were identified. Little linkage was found among phenotypic and rep-polymerase chain reaction (rep-PCR) genomic fingerprinting profiles of the pathogens except at the species/pathovar level; strains displaying virtually identical fingerprint profiles were found to correspond to distinct races and vice versa. The rep-PCR genomic fingerprinting analyses suggest that certain lineages may have evolved or predominated in specific regions or specific countries.