Reduced gap strains induce changes in bone regeneration during distraction.
Reduced gap strains induce changes in bone regeneration during distraction.
复制标题
间隙应变的减少会导致注意力分散期间骨再生的变化。
DOI：
10.1115/1.2798331
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
Goulet,JA
中科院分区：
文献类型：
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作者：
Richards,M;Waanders,NA;Weiss,JA;Bhatia,V;Senunas,LE;Schaffler,MB;Goldstein,SA;Goulet,JA
采用新西兰白色兔双侧肢体牵张成骨模型，研究牵张成骨过程中应变环境与骨再生的关系。在7只（n = 7）家兔中，在固定器一侧应用加强件，以减少延长完成后差距组织内的应变。6天后对动物实施安乐死，收获其牵引区并分析新骨体积和结构的变化。进行非线性有限元分析（FEA）以预测差距应变环境的变化。有限元分析结果预测，牵引区内的平均应变测量值几乎均匀降低7倍。与对侧对照组相比，在间隙应变实验性降低的实验组中，观察到总平均新骨体积无变化，骨体积分数（BV/TV）和骨小梁厚度（Tb.Th）显著降低。这些结果表明，固定器硬化影响了结构，但不影响新骨形成的数量。这种牵张的动物模型可用于研究应变场影响骨修复和再生事件的机制，如细胞增殖、前体组织分化以及改变生长因子和营养物质向组织的输送。
A bilateral New Zealand white rabbit model of distraction osteogenesis (DO) was used to investigate the relationship between strain environment and bone regeneration during limb lengthening. In seven (n = 7) rabbits, a stiffener was applied to the fixator on one side to reduce strains within the gap tissue after lengthening was completed. Animals were euthanized six days later and their distraction zones were harvested and analyzed for changes in new bone volume and architecture. Nonlinear finite element analyses (FEA) were performed to predict changes in the gap strain environment. FEA results predicted a nearly uniform sevenfold decrease in average strain measures within the distraction zone. No change in total average new bone volume and significant decreases in both bone volume fraction (BV/TV) and trabecular thickness (Tb.Th) were observed in tibiae in which gap strains were reduced experimentally, compared to contralateral controls. These results suggest that fixator stiffening influenced the architecture but not the amount of newly formed bone. This animal model of distraction might be used to study the mechanisms by which strain fields affect events in bone repair and regeneration, such as cell proliferation, precursor tissue differentiation, and altered growth factor and nutrient delivery to tissues.