Multilocus sequence typing system for Campylobacter jejuni
Multilocus sequence typing system for Campylobacter jejuni
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DOI：
10.1128/jcm.39.1.14-23.2001
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发表时间：
2001-01-01
影响因子：
9.4
通讯作者：
Maiden, MCJ
中科院分区：
文献类型：
--
作者：
Dingle, KE;Colles, FM;Maiden, MCJ
革兰氏阴性细菌空肠弯曲菌在牲畜和环境中具有广泛的储存库，是人类胃肠炎的常见原因。迄今为止，缺乏（i）适合群体遗传分析的方法和（II）普遍接受的命名法阻碍了对该生物体的流行病学和群体生物学的研究。在此，描述了该生物体的多位点序列分型 (MLST) 系统，该系统利用七个管家基因座中存在的遗传变异来确定分离株之间的遗传关系。MLST 系统是使用来自人类、动物和环境的不同来源的 194 个空肠弯曲菌分离株建立的。这些分离株的等位基因图谱或序列类型 (ST) 存放在互联网 (http://mlst.zoo.ox.ac.uk) 上，形成一个可以不断扩展的虚拟分离株集合。这些数据表明，空肠弯曲菌具有遗传多样性，具有弱克隆群体结构，并且种内和种间水平遗传交换很常见。在观察到的 155 个 ST 中，有 51 个（占分离株集合的 26%）是独特的，集合的其余部分被分为 11 个相关 ST 谱系或克隆复合体，成员数在 2 到 56 之间。在某些情况下，给定谱系或 ST 的成员资格与特定 Penner IIS 血清型的拥有相关，应用这种方法进一步分离收集将能够建立空肠弯曲菌流行病学的综合全球图景，并将允许对该生物体的群体遗传学进行更详细的研究。
The gram-negative bacterium Campylobacter jejuni has extensive reservoirs in livestock and the environment and is a frequent cause of gastroenteritis in humans. To date, the lack of (i) methods suitable for population genetic analysis and (II) a universally accepted nomenclature has hindered studies of the epidemiology and population biology of this organism. Here, a multilocus sequence typing (MLST) system for this organism is described, which exploits the genetic variation present in seven housekeeping loci to determine the genetic relationships among isolates, The MLST system was established using 194 C. jejuni isolates of diverse origins, from humans, animals, and the environment. The allelic profiles, or sequence types (STs), of these isolates were deposited on the Internet (http://mlst.zoo.ox.ac.uk), forming a virtual isolate collection which could be continually expanded. These data indicated that C. jejuni is genetically diverse, with a weakly clonal population structure, and that intra- and interspecies horizontal genetic exchange was common. Of the 155 STs observed, 51 (26% of the isolate collection) were unique, with the remainder of the collection being categorized into 11 lineages or clonal complexes of related STs with between 2 and 56 members. In some cases membership in a given lineage or ST correlated with the possession of a particular Penner IIS serotype, Application of this approach to further isolate collections will enable an integrated global picture of C. jejuni epidemiology to be established and will permit more detailed studies of the population genetics of this organism.