Beta-Amyloid-Dependent miRNAs as Circulating Biomarkers in Alzheimer's Disease: a Preliminary Report
Beta-Amyloid-Dependent miRNAs as Circulating Biomarkers in Alzheimer's Disease: a Preliminary Report
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DOI：
10.1007/s12031-020-01511-0
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发表时间：
2020-04-18
影响因子：
3.1
通讯作者：
Mehdizadeh, Mehdi
中科院分区：
文献类型：
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作者：
Hajjri, Seyedeh Nazanin;Sadigh-Eteghad, Saeed;Mehdizadeh, Mehdi
microRNA（miRNAs）由于其调节性质而被认为是诊断和预测阿尔茨海默病（AD）的最可靠的生物标志物之一。本研究的主要目的是评估miR 4422和miR 3714的表达，作为GSAP和BACE 1表达的主要调节因子，与健康受试者相比，在AD患者中。20例轻度至中度AD患者（58-71岁）和15例健康受试者（58-73岁）参加了本研究。检测两组中靶基因miR 4422和miR 3714的表达水平，参照基因5S rRNA和miRlet 7a-5 p的表达水平。为了比较病例组和对照组之间的表达，根据数据分布模式使用t检验或Wilcoxon检验。miR 4422、miR 3714、5S rRNA和miRlet 7a-5 p基因的扩增效率均在可接受的范围内。两组之间的平均miR 4422 -5S rRNA dCt值存在显著差异（p = 0.018）。表达的相对倍数变化为0.43。两组之间的平均miR 4422-miRlet 7a-5 p dCt值（p = 0.41）、平均miR 3714 -5S rRNA dCt值（p = 0.10）和平均miR 3714-miRlet 7a-5 p dCt值（p = 0.063）无显著差异。我们表明miR 4422可能是阿尔茨海默病诊断的可靠生物标志物。看来，靶向GSAP和BACE 1表达的miR 4422的表达减少可能会导致A β斑块的形成增加。
MicroRNAs (miRNAs) are considered among the most reliable biomarkers to diagnose and predict Alzheimer's disease (AD), due to their regulatory nature. The main goal of this study was to evaluate the expression of miR4422 and miR3714, as the main regulators of GSAP and BACE1 expression, in AD patients compared with healthy subjects. Twenty patients with a mild to moderate AD (58-71 years old) and 15 healthy subjects (58-73 years old) participated in this study. The expression levels of miR4422 and miR3714 as the target genes and 5S rRNA and miRlet7a-5p as the reference genes were measured in the two groups. To compare the expression between the case and the control groups, the t test or the Wilcoxon test was used, based on the data distribution patterns. The efficiencies of amplification of the miR4422, miR3714, 5S rRNA, and miRlet7a-5p genes all were in the acceptable range. The mean miR4422-5S rRNA dCt value was significantly different between the two groups (p = 0.018). The relative fold change of the expression was 0.43. The mean miR4422-miRlet7a-5p dCt value (p = 0.41), the mean miR3714-5S rRNA dCt value (p = 0.10), and the mean miR3714-miRlet7a-5p dCt value (p = 0.063) were not significantly different between the two groups. We indicated that miR4422 could be a reliable biomarker for Alzheimer's diagnosis. It seems that the reduced expression of miR4422 that targets GSAP and BACE1 expression can lead to an increase in the formation of A beta plaque.