MODE OF ACTION OF THE MASSIVELY ACCUMULATED BETA-CAROTENE OF DUNALIELLA-BARDAWIL IN PROTECTING THE ALGA AGAINST DAMAGE BY EXCESS IRRADIATION
MODE OF ACTION OF THE MASSIVELY ACCUMULATED BETA-CAROTENE OF DUNALIELLA-BARDAWIL IN PROTECTING THE ALGA AGAINST DAMAGE BY EXCESS IRRADIATION
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DOI：
10.1104/pp.91.3.1040
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发表时间：
1989-11-01
期刊：
影响因子：
7.4
通讯作者：
AVRON, M
中科院分区：
文献类型：
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作者：
BENAMOTZ, A;SHAISH, A;AVRON, M
当在确定的条件下生长时，杜氏藻积累高浓度的β-胡萝卜素，其主要由两种异构体全反式和9-顺式β-胡萝卜素组成。胡萝卜素高β-与低β-胡萝卜素相比，胡萝卜素D bardawil或杜氏盐藻（Dunaliella salina），其不能积累β-胡萝卜素在高β-受体中对抗光抑制的保护胡萝卜素D当蓝光用作光抑制剂时，Bardawil非常强，与白色光中等，与红光不存在。这些观察表明，大量积累的β-胡萝卜素在D. Bardawil通过吸收光谱的蓝色区域来屏蔽，从而保护细胞免受高辐射的损害。辐照D.通过高强度蓝光的Bardawil导致以下事件的时间顺序：氧释放的光抑制，9-顺式β-葡聚糖的光破坏，胡萝卜素、全反式β-胡萝卜素、叶绿素的光破坏和细胞死亡。
When grown under defined conditions Dunaliella bardawil accumulates a high concentration of .beta.-carotene, which is composed primarily of two isomers, all-trans and 9-cis .beta.-carotene. The high .beta.-carotene alga is substantially resistant to photoinhibition of photosynthetic oxygen evolution when compared with low .beta.-carotene D. bardawil or with Dunaliella salina which is incapable of accumulating .beta.-carotene. Protection against photoinhibition in the high .beta.-carotene D. bardawil is very strong when blue light is used as the photoinhibitory agent, intermediate with white light, and nonexistent with red light. These observations suggest that the massively accumulated .beta.-carotene in D. bardawil protects the alga against damage by high irradiation by screening through absorption of the blue region of the spectrum. Irradiation of D. Bardawil by high intensity blue light results in the following temporal sequence of events: photoinhibition of oxygen evolution, photodestruction of 9-cis .beta.-carotene, photodestruction of all-trans .beta.-carotene, photodestruction of chlorophyll and cell death.