Regulation of interferon action in human fibroblasts: transient induction of specific proteins and amplification of the antiviral response by antinomycin D.
Regulation of interferon action in human fibroblasts: transient induction of specific proteins and amplification of the antiviral response by antinomycin D.
复制标题
人成纤维细胞中干扰素作用的调节：特定蛋白质的瞬时诱导和抗霉素 D 抗病毒反应的放大。
DOI：
10.1016/0042-6822(81)90337-8
复制
发表时间：
1981
期刊：
影响因子：
3.7
通讯作者：
Holmes,SL
中科院分区：
文献类型：
--
作者：
Gupta,SL;Rubin,BY;Holmes,SL
我们早先报道过干扰素处理人成纤维细胞诱导了许多蛋白质，并且这种诱导似乎与抗病毒状态的发展相关。干扰素诱导的蛋白质的积累在干扰素处理后几小时内达到最大值，然后显著下降，尽管培养基中继续存在干扰素，表明这种诱导可能受到调节。发现RNA合成抑制剂（放线菌素D、5，6-二氯-1-β-d-呋喃核糖基苯并咪唑）对干扰素的抗病毒作用具有双重作用。当与干扰素一起加入时，它们阻止了抗病毒状态的发展。相反，当在较晚的时间间隔（干扰素后4-8小时）加入时，抗病毒效果大大增强。这种扩增与各种干扰素诱导的蛋白质水平和2′，5 ′-寡腺苷酸合成酶活性的增加有关。结果表明，细胞对干扰素的反应是受调节的，可以操纵。证据也表明，干扰素诱导的基因转录所需的发展的抗病毒状态可能会延长几个小时。
We reported earlier that interferon treatment of human fibroblasts induces a number of proteins, and that this induction seems to correlate with the development of the antiviral state. The accumulation of interferon-induced proteins reaches a maximum within a few hr after interferon treatment and is then followed by a marked decline despite the continued presence of interferon in the medium indicating that this induction may be subject to regulation. Inhibitors of RNA synthesis (actinomycin D, 5,6-dichloro-1-β-d-ribofuranosylbenzimidazole) were found to have a dual effect on the antiviral action of interferon. When added together with interferon, they blocked the development of the antiviral state. In contrast, when added at later intervals (4–8 hr after interferon), the antiviral effect was greatly amplified. This amplification was associated with increased levels of various interferon-induced proteins and 2′,5′-oligoadenylate synthetase enzyme activity. The results indicate that the cellular response to interferon is subject to regulation and can be manipulated. Evidence is also presented which indicates that the interferon-induced genetic transcription required for the development of the antiviral state may extend for several hours.