A customized contamination controlling approach for culturing oleaginous Nannochloropsis oceanica
A customized contamination controlling approach for culturing oleaginous Nannochloropsis oceanica
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用于培养产油微拟球藻的定制污染控制方法
DOI：
10.1016/j.algal.2017.07.013
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发表时间：
2017-11-01
影响因子：
5.1
通讯作者：
Lu, Yandu
中科院分区：
文献类型：
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作者：
Gan, Qinhua;Zhou, Wenxu;Lu, Yandu
微藻的功能基因组学研究取得了巨大的进展，对藻类养殖技术的发展产生了巨大的影响，但如何合理利用藻类基因组来发现藻类养殖污染的防治策略还有待探索。通过真菌和微藻的基因组比较，我们可以发现杀菌剂作用靶标甾醇生物合成途径中关键酶的差异和遗传结构。通过进一步筛选和表征针对选定的甾醇生物合成酶的化学抑制剂，开发了针对产油海洋微拟球藻的种特异性污染控制方法。在低至1 μ g/ml的浓度下，triticonazole，一种甾醇14-脱甲基酶抑制剂，能够有效地消除污染的真菌和潜在的入侵藻类菌株，并能够刺激所需微藻物种的生长。这些发现表明，跨界基因组比较有助于识别遗传差异，其可用于选择合适的和定制的微生物污染控制方法，以培养具有所需生产性状的个体微藻物种。
Functional genomics of microalgae have undergone tremendous progress and have a huge impact for streamlining the algal cultivation technology; however, the rational utilization of algal genomes for discovering the strategies to combat algal culture contamination remain unexplored. Genome comparison of fungi and microalgae allowed us to find insights into the possible distinctions of key enzymes and genetic structural of sterol biosynthetic pathway, which are the targets for commercial fungicides. By further screening and characterizing chemical inhibitors targeting selected sterol biosynthetic enzymes, a species-specific contamination control method tailored to oleaginous Nannochloropsis oceanica has been developed. With a concentration of as low as 1 mu g/ml, triticonazole, a sterol 14-demethylase inhibitor, was capable of effectively eliminating contaminated fungi and potential invasive algal strains, and able to stimulate the growth of desired microalgal species. These findings suggest that the crossing kingdom genome comparison helps to identify genetic distinctions which can be utilized to select appropriate and customized microbial contamination control approach for culturing individual microalgal species with desired production traits.