Normal function of neonatal polymorphonuclear leukocytes in antibody-dependent cellular-cytotoxicity to herpes simplex virus-infected cells.
Normal function of neonatal polymorphonuclear leukocytes in antibody-dependent cellular-cytotoxicity to herpes simplex virus-infected cells.
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新生儿多形核白细胞对单纯疱疹病毒感染细胞的抗体依赖性细胞毒性的正常功能。
DOI：
10.1016/s0022-3476(81)80847-5
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发表时间：
1981
期刊：
影响因子：
--
通讯作者：
Loo,LS
中科院分区：
文献类型：
--
作者：
Kohl,S;Frazier,JP;Pickering,LK;Loo,LS
材料和方法目标细胞为Chang肝细胞，在使用前24小时感染HSV I型(HE株)，并在使用当天用放射性铬标记，如前所述。在足月婴儿出生后，同时从脐带静脉的胎盘部分获取血液，并在适当情况下从患者或父母签署知情同意后从健康成人对照组获取血液。肝素化血液(10单位/毫升)经顺序葡聚糖沉淀和
MATERIALS AND METHODSTarget cells were Chang liver ceils, infected with HSV type I (HE strain) 24 hours prior to use, and labeled with radioactive chromium the day of use, as previously described in detail.-'.:'.'Blood was obtained simultaneously from the placental portion of the umbilical vein after delivery of term infants, and from healthy adult controls, after signed informed consent from patients or parents, as appropriate. Heparinized blood (10 units/ml) was processed by sequential dextran sedimentation and