Oscillatory cAMP cell-cell signalling persists during multicellular Dictyostelium development.
Oscillatory cAMP cell-cell signalling persists during multicellular Dictyostelium development.
复制标题
振荡 cAMP 细胞间信号传导在多细胞盘基网柄菌发育过程中持续存在。
DOI：
10.1038/s42003-019-0371-0
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发表时间：
2019
影响因子：
5.9
通讯作者：
Singer G
中科院分区：
文献类型：
--
作者：
Singer G
cAMP的传播波，周期性地在聚集中心启动，被认为可以引导成千上万饥饿的盘状体盘状细胞趋化聚集成多细胞聚集体。传播光密度波，反映细胞的周期性运动，已经被证明存在于流动的聚集体，土丘和迁移的鼻涕虫中。使用高灵敏度的cAMP- fret报告器，我们现在已经能够直接测量这些多细胞结构中周期性传播的cAMP波。在鼻涕虫中，cAMP波周期性地在尖端开始，并通过孢子前区向后传播。发育缺陷突变体中cAMP信号动力学的改变有力地支持了cAMP波在多细胞盘状骨形态发生中的关键功能作用。这些发现表明，扩殖性cAMP不仅控制了初始聚集过程，而且在土墩和鼻涕虫阶段的多细胞盘形骨形成过程中仍然是指导细胞运动的长期细胞间通信机制。
Propagating waves of cAMP, periodically initiated in the aggregation centre, are known to guide the chemotactic aggregation of hundreds of thousands of starving individualDictyostelium discoideumcells into multicellular aggregates. Propagating optical density waves, reflecting cell periodic movement, have previously been shown to exist in streaming aggregates, mounds and migrating slugs. Using a highly sensitive cAMP-FRET reporter, we have now been able to measure periodically propagating cAMP waves directly in these multicellular structures. In slugs cAMP waves are periodically initiated in the tip and propagate backward through the prespore zone. Altered cAMP signalling dynamics in mutants with developmental defects strongly support a key functional role for cAMP waves in multicellular Dictyostelium morphogenesis. These findings thus show that propagating cAMP not only control the initial aggregation process but continue to be the long range cell-cell communication mechanism guiding cell movement during multicellularDictyosteliummorphogenesis at the mound and slugs stages.