Comparison of morphological changes in efferent lymph nodes after implantation of resorbable and non-resorbable implants in rabbits.
Comparison of morphological changes in efferent lymph nodes after implantation of resorbable and non-resorbable implants in rabbits.
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DOI：
10.1186/1475-925x-10-32
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发表时间：
2011-04-26
影响因子：
3.9
通讯作者：
Meyer-Lindenberg A
中科院分区：
文献类型：
--
作者：
Bondarenko A;Hewicker-Trautwein M;Erdmann N;Angrisani N;Reifenrath J;Meyer-Lindenberg A
近年来，镁合金作为可生物降解的植入材料引起了人们的广泛兴趣。已知植入物降解产物可诱导几种类型的免疫应答。因此，本研究的目的是检查植入不同可吸收镁合金（MgCa0.8，LAE 442）后传出淋巴结的形态学变化，并与市售可吸收（PLA）和不可吸收（钛）植入材料以及不含植入材料的对照组进行比较。将不同的植入材料髓内插入兔胫骨。术后观察3个月和6个月后，对腘淋巴结进行组织学和免疫组织学检查，并与假手术动物和未手术动物的淋巴结进行比较。苏木精和伊红染色用于细胞分化。小鼠抗CD79 α和大鼠抗CD3单克隆一抗用于B和T淋巴细胞检测，小鼠抗CD68一抗用于巨噬细胞检测。采用半定量评分对所有切片进行评价。组织学评价表明，两种镁合金（LAE442和MgCa0.8）的形态学变化水平为低和中等。钛在窦组织细胞增生症和组织细胞凋亡（3个月）中以及PLA在组织细胞凋亡（3个月和6个月）中达到高于中等值。对所有研究植入物的免疫反应具有非特异性特征，主要是异物反应。LAE442引起的变化最小，这可能是由于与MgCa0.8相比降解速率较低。因此，它是具有低免疫原性潜力的植入物的有希望的候选物。与广泛使用的植入材料钛和PLA相比，两种检查的镁合金均未引起传出淋巴结的形态学变化显著增加。LAE442诱导甚至更低的免疫反应。因此MgCa0.8和特别是LAE442是生物医学用途的合适候选物。
Magnesium alloys as biodegradable implant materials received much interest in recent years. It is known that products of implant degradation can induce several types of immune response. Hence, the aim of this study was to examine the morphological changes of efferent lymph nodes after implantation of different resorbable magnesium alloys (MgCa0.8, LAE442) in comparison to commercially available resorbable (PLA) and non-resorbable (titanium) implant materials as well as control groups without implant material. The different implant materials were inserted intramedullary into the rabbit tibia. After postoperative observation periods of three and six months, popliteal lymph nodes were examined histologically and immunhistologically and compared to lymph nodes of sham operated animals and animals without surgery. Haematoxylin and eosin staining was performed for cell differentiation. Mouse anti-CD79α and rat anti-CD3 monoclonal primary antibodies were used for B- and T-lymphocyte detection, mouse anti-CD68 primary antibodies for macrophage detection. Evaluation of all sections was performed applying a semi quantitative score. The histological evaluation demonstrated low and moderate levels of morphological changes for both magnesium alloys (LAE442 and MgCa0.8). Higher than moderate values were reached for titanium in sinus histiocytosis and histiocytic apoptosis (3 months) and for PLA in histiocytic apoptosis (3 and 6 months). The immune response to all investigated implants had a non-specific character and predominantly was a foreign-body reaction. LAE442 provoked the lowest changes which might be due to a lower degradation rate in comparison to MgCa0.8. Therewith it is a promising candidate for implants with low immunogenic potential. Both examined magnesium alloys did not cause significantly increased morphological changes in efferent lymph nodes in comparison to the widely used implant materials titanium and PLA. LAE442 induced even lower immunological reactions. Therewith MgCa0.8 and especially LAE442 are appropriate candidates for biomedical use.