Direct electron transfer with enzymes on nanofiliform titanium oxide films with electron-transport ability.
Direct electron transfer with enzymes on nanofiliform titanium oxide films with electron-transport ability.
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DOI：
10.1016/j.bios.2012.08.037
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发表时间：
2013-03
影响因子：
12.6
通讯作者：
So-Yoon Lee;Ryosuke Matsuno;K. Ishihara;M. Takai
中科院分区：
文献类型：
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作者：
So-Yoon Lee;Ryosuke Matsuno;K. Ishihara;M. Takai
生物分子与电极之间的直接电子转移（DET）是一个不需要电子介质的过程，因此，为了在不受任何介质干扰的情况下监测电极与生物分子之间的电子转移，需要有上级的选择性和灵敏度。然而，DET是困难的，因为氧化还原中心是蛋白质如酶的电子活性中心被深埋。因此，到达氧化还原中心的独特电极纳米结构是一个关键因素。在这里，我们系统地研究了使用各种nanofiliformed电极形态和酶浓度的DET术语。指出反应位点在100 nm以下，单位表面积上的酶吸附量在1.0以下是影响DET的主要因素。作为一个很好的应用，我们开发了一种生物传感器直接监测过氧化氢（H2 O2）的检测能力从过氧化物酶。对于所制备的HRP/nTOF/Ti电极，观察到的催化电流值与H2 O2浓度的增加呈线性关系，直到100μM，这表明H2 O2生物传感器的良好潜力。
Direct electron transfer (DET) from biomolecules to electrode is a process without electron-mediators, thus superior selectivity and sensitivity is expected in order to monitor electron transfer between electrode and biomolecules without any mediator interference. However, DET is difficult because a redox center which is an electron active center of proteins such as enzymes is buried deep. So, a unique electrode nanostructure to reach the redox center is a critical factor. Here we have systematically investigated terms for DET using various nanofiliformed electrode morphologies and enzyme concentrations. It is pointed out that the reaction site is below 100nm, the ration amounts of adsorbed enzyme per surface area are below 1.0 are contributed to the DET. As a great application, we have developed a biosensor monitoring the hydrogen peroxide (H2O2) detecting capability from peroxidase directly. For the fabricated HRP/nTOF/Ti-electrodes observed the catalytic current value was linear according to the increase in the concentration of H2O2up to 100μM, which indicates a good potential for an H2O2biosensor.