Protein Adsorption to Titanium and Zirconia Using a Quartz Crystal Microbalance Method.
Protein Adsorption to Titanium and Zirconia Using a Quartz Crystal Microbalance Method.
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DOI：
10.1155/2017/1521593
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发表时间：
2017
影响因子：
--
通讯作者：
Hayakawa T
中科院分区：
文献类型：
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作者：
Kusakawa Y;Yoshida E;Hayakawa T
用27 MHz石英晶体微天平研究了蛋白质在钛(Ti)和氧化锆(ZrO2)上的吸附行为。以细胞黏附蛋白纤维连接蛋白(Fn)和细胞黏附抑制蛋白白蛋白(Alb)为研究对象。通过原子力显微镜和电子探针显微分析观察、与水的接触角测量和表面粗糙度的测量，对QCM用的钛和氧化锆传感器进行了表征。通过QCM测量，得到了FN和Alb在Ti和ZrO2传感器上的吸附量和表观反应速率。钛传感器对FN和Alb的吸附能力强于ZrO2传感器。此外，FN在钛或氧化锆传感器上的吸附量高于Alb。表面粗糙度和表面亲水性可能会影响FN或Alb的吸附。就吸附速率而言，Alb在钛表面的吸附速度比FN快。相比于钛和氧化锆，Alb对钛的吸附速度要快于对二氧化锆的吸附。FN吸附对细胞活性有效，而Alb吸附无效。QCM方法可以在体内模拟FN和Alb在Ti或ZrO2上的吸附。
Protein adsorption onto titanium (Ti) or zirconia (ZrO2) was evaluated using a 27 MHz quartz crystal microbalance (QCM). As proteins, fibronectin (Fn), a cell adhesive protein, and albumin (Alb), a cell adhesion-inhibiting protein, were evaluated. The Ti and ZrO2 sensors for QCM were characterized by atomic force microscopy and electron probe microanalysis observation, measurement of contact angle against water, and surface roughness. The amounts of Fn and Alb adsorbed onto the Ti and ZrO2 sensors and apparent reaction rate were obtained using QCM measurements. Ti sensor showed greater adsorption of Fn and Alb than the ZrO2 sensor. In addition, amount of Fn adsorbed onto the Ti or ZrO2 sensors was higher than that of Alb. The surface roughness and hydrophilicity of Ti or ZrO2 may influence the adsorption of Fn or Alb. With regard to the adsorption rate, Alb adsorbed more rapidly than Fn onto Ti. Comparing Ti and ZrO2, Alb adsorption rate to Ti was faster than that to ZrO2. Fn adsorption will be effective for cell activities, but Alb adsorption will not. QCM method could simulate in vivo Fn and Alb adsorption to Ti or ZrO2.