Intracellular pH is increased after transformation of Chinese hamster embryo fibroblasts.
Intracellular pH is increased after transformation of Chinese hamster embryo fibroblasts.
复制标题
中国仓鼠胚胎成纤维细胞转化后细胞内pH值升高。
DOI：
10.1073/pnas.84.9.2766
复制
发表时间：
1987
影响因子：
11.1
通讯作者：
Pardee,AB
中科院分区：
文献类型：
--
作者：
Ober,SS;Pardee,AB
这些研究表明，一系列致瘤性中国仓鼠胚胎成纤维细胞（CHEF）细胞系保持的内部pH值（pHi）比非致瘤性CHEF/18亲本细胞系高0.12 +/- 0.04 pH单位。致瘤性CHEF细胞中pHi的增加不是由于自分泌生长因子的产生或先前显示调节CHEF/18系中存在的Na+/H+-反向转运蛋白活性的途径的持续活化。这些发现表明，致瘤性CHEF/18衍生物中pHi调节的缺陷在于Na+/H+反向转运蛋白本身。进一步的研究，以确定增加的pHi的生物学意义表明，外部pH值（pHo）的依赖性曲线启动的DNA合成的致瘤CHEF线移动约0.2 pH值单位向酸性值相对于nontumorigenic CHEF/18的父母。这些数据表明，在中国仓鼠胚胎成纤维细胞的DNA合成的调节中，pHi的关键作用，并证明，在pHi的畸变可以有助于收购改变生长特性。
These studies reveal that a series of tumorigenic Chinese hamster embryo fibroblast (CHEF) cell lines maintain an internal pH (pHi) that is 0.12 +/- 0.04 pH unit above that of the nontumorigenic CHEF/18 parental line. This increase of pHi in the tumorigenic CHEF cells is not due to autocrine growth factor production or to the persistent activation of pathways previously shown to modulate Na+/H+-antiporter activity present in the CHEF/18 line. These findings suggest that the defect in pHi regulation in the tumorigenic CHEF/18 derivatives lies in the Na+/H+ antiporter itself. Further studies to determine the biological significance of an increased pHi show that the external pH (pHo)-dependence curve for initiation of DNA synthesis in the tumorigenic CHEF lines is shifted by approximately 0.2 pH unit toward acidic values relative to that of the nontumorigenic CHEF/18 parent. These data show a critical role for pHi in the regulation of DNA synthesis in Chinese hamster embryo fibroblasts and demonstrate that aberrations in pHi can contribute to the acquisition of altered growth properties.