THE ASSOCIATION OF LIPOPROTEINS WITH THE INHIBITION OF STREPTOLYSIN-S BY SERUM
THE ASSOCIATION OF LIPOPROTEINS WITH THE INHIBITION OF STREPTOLYSIN-S BY SERUM
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DOI：
10.1172/jci102408
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发表时间：
1950-01-01
影响因子：
15.9
通讯作者：
MACLEOD, CM
中科院分区：
文献类型：
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作者：
STOLLERMAN, GH;BERNHEIMER, AW;MACLEOD, CM
链球菌溶血素S的抑制是由于血清的正常成分，而不是特异性抗体。多种正常动物血清。抑制链球菌溶血素S，这种抑制作用不依赖于人、兔和马血清中的γ球蛋白。家兔注射链球菌溶血素S后未出现特异性抗体。用链球菌溶血素S或用β-溶血性链球菌的活培养物。最高程度的链球菌溶血素S抑制出现在与α-1和β-1脂蛋白相关的血清组分中，当血清通过冷酒精法分级分离时。当血清通过用硫酸铵盐析而分级分离时，白蛋白级分也与链球菌溶血素S抑制相关。磷脂（特别是卵磷脂）的盐水悬浮液可抑制链球菌溶血素S。当血清用魏氏梭菌卵磷脂酶或醚或醚-醇混合物处理时，链球菌溶血素S抑制作用显著降低。胰蛋白酶消化、加热至凝固点附近、在4 ℃或4 ℃以上长期储存以及用硫酸铵分级分离可增加血清对链球菌溶血素S的抑制作用。用硫酸铵分级分离后，马血清的总抑制活性提高了3.75倍，人血清提高了2.41倍。在每种情况下，用卵磷脂酶或用乙醚处理产生链球菌溶血素S抑制的显著降低。血清中的链球菌溶血素S抑制剂似乎由磷脂蛋白复合物组成，血清中的磷脂可能在其稳定性及其组成中发挥作用。
The inhibition of streptolysin S is due to normal components of serum rather than to a specific antibody. The sera of a wide variety of normal animal spp. inhibit streptolysin S and this inhibition is independent of gamma globulins in the sera of humans, rabbits and horses. Specific antibodies to streptolysin S did not appear when rabbits were injd. with streptolysin S or with live cultures of beta-hemolytic streptococci. The highest degree of strepto-lysin S inhibition appears in the serum fractions associated with alpha-1 and beta-1 lipoproteins when serum is fractionated by the cold alcohol method. When serum is fractionated by salting out with ammonium sulfate, the albumin fraction is also associated with streptolysin S inhibition. Saline suspensions of phospholipids, particularly lecithin, inhibit streptolysin S. A marked reduction of streptolysin S inhibition occurs when serum is treated with Clostridium welchii lecithinase, or with ether or ether-alcohol mixture. Inhibition of streptolysin S by serum is increased by tryptic digestion, by heating near the coagulation point, by prolonged storage at or above 4[degree]C, and by fractionation with ammonium sulfate. Following fractionation with ammonium sulfate, the increase in total inhibitory activity of horse serum was 3.75 times, and of human serum 2.41 times. In each instance, treatment with lecithinase or with ether produces a marked decrease in streptolysin S inhibition. The streptolysin S inhibitor in serum appears to be composed of a phospholipo-protein complex and the phospholipids of serum may play a role in its stabilization as well as in its composition.