Establishment and characterization of human lingual squamous cell carcinoma cell lines designated Nialym derived from metastatic foci of lymph node, and Nialymx derived from transplanted tumor of Nialym cells
Establishment and characterization of human lingual squamous cell carcinoma cell lines designated Nialym derived from metastatic foci of lymph node, and Nialymx derived from transplanted tumor of Nialym cells
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人舌鳞状细胞癌细胞系的建立和表征，命名为源自淋巴结转移灶的 Nialym 和源自 Nialym 细胞移植肿瘤的 Nialymx
DOI：
10.1007/s13577-014-0107-x
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发表时间：
2015
期刊：
影响因子：
4.3
通讯作者：
Tanaka A
中科院分区：
文献类型：
--
作者：
Takahashi H;Ishikawa H;Mataga I;Tanaka A
鳞状细胞癌细胞系Nialym是从一位48岁的日本男性舌鳞癌患者的淋巴结转移灶中成功建立的。此外，从SCID小鼠的Nialym细胞移植瘤中建立了Nialymx细胞系。Nialym细胞呈棱角状，具有肿瘤性和多形性特征。电子显微镜下可见两种类型的Nialym细胞：亮细胞和暗细胞。暗细胞胞质内有许多波浪形的张力细丝，而亮细胞细胞器发育不良。在第10代时，Nialym和Nialymx细胞的群体倍增时间分别约为46和42小时。Nialym细胞可分泌4.8 ng/mlVEGF和5.9 ng/mlHGF；第10代培养3d时，Nialymx细胞也可分泌6.7 ng/mlVEGF和4.3 ng/mlHGF。Nialym和Nialymx细胞系的组织病理学特征相似。我们认为，这些细胞系是阐明癌症转移机制和开发免疫治疗和化疗方案的有价值的工具。
The squamous cell carcinoma cell lines Nialym was successfully established from metastatic foci of lymph nodes from a 48-year-old male Japanese patient with squamous cell carcinoma of the tongue. In addition, the Nialymx cell line was established from a transplanted tumor of Nialym cells in SCID mice. Nialym cells were angular, with neoplastic and pleomorphic features. Two types of Nialym cell were observed by electron microscopy; light cells and dark cells. The dark cells had a number of waved tonofilaments in the cytoplasm, while light cells showed poorly developed organelles. The population doubling times for Nialym and Nialymx cells were approximately, 46 and 42 h at the 10th passage. Nialym cells secreted 4.8 ng/ml VEGF and 5.9 ng/ml HGF, Nialymx cells also secreted 6.7 ng/ml VEGF and 4.3 ng/ml HGF at the 10th passage for 3 days of culture. Histopathological aspects of Nialym and Nialymx cell lines were similar. We believe that these cell lines are valuable tools for elucidating the mechanisms of cancer metastasis and developing immunotherapy and chemotherapy regimens.