Genetic interactions between an RNA polymerase II phosphatase and centromeric elements in Saccharomyces cerevisiae.
Genetic interactions between an RNA polymerase II phosphatase and centromeric elements in Saccharomyces cerevisiae.
复制标题
RNA 聚合酶 II 磷酸酶和酿酒酵母着丝粒元件之间的遗传相互作用。
DOI：
10.1007/s00438-004-1009-5
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发表时间：
2004
期刊：
影响因子：
--
通讯作者：
Kane,CM
中科院分区：
文献类型：
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作者：
Pierstorff,E;Kane,CM
酿酒酵母蛋白磷酸酶 Fcp1 参与 RNA 聚合酶 II 的转录调节，由必需基因 FCP1 编码。对两种磷酸酶突变体fcp1-2和fcp1-4的温度敏感表型的多拷贝抑制子进行了筛选。仅发现野生型 FCP1 能够抑制（互补）fcp1-4 突变。对于fcp1-2，鉴定了三个第二位点抑制子。其中一个包含 ZDS1 的 ORF。其余两个抑制子映射到染色体 I 和 V 的着丝粒区域。发现由着丝粒 DNA 引起的抑制比着丝粒的其他区域更依赖于 CDEIII 区域。抑制着丝粒的存在影响 fcp1-2 细胞中 Fcp1 蛋白的水平和 RNA 聚合酶 II (RNAPII) 的整体磷酸化状态，但不影响在允许和非允许温度下生长的野生型细胞。此外，还鉴定了该 FCP1 突变体与编码着丝粒结合蛋白的基因 SKP1、CEP3 和 CBF1 之间的遗传相互作用。讨论了着丝粒DNA抑制和调节Fcp1-2蛋白活性的机制。
TheSaccharomyces cerevisiaseprotein phosphatase Fcp1 has been implicated in the regulation of transcription by RNA polymerase II, and is encoded by the essential geneFCP1. A screen was carried out for multicopy suppressors of the temperature-sensitive phenotype of two phosphatase mutants,fcp1-2andfcp1-4. Only the wild-typeFCP1was found to suppress (complement) thefcp1-4mutation. Forfcp1-2three second-site suppressors were identified. One contained the ORF forZDS1. The remaining two suppressors mapped to the centromere regions of chromosomes I and V. Suppression due to centromere DNA was found to be more dependent on the CDEIII region than on other regions of the centromere. The presence of a suppressor centromere affected the level of Fcp1 protein and the overall phosphorylation state of RNA polymerase II (RNAPII) infcp1-2cells, but not wild-type cells, grown at both permissive and non-permissive temperatures. In addition, genetic interactions were identified between thisFCP1mutant and the genesSKP1,CEP3andCBF1, which code for centromere binding proteins. The mechanism of suppression and regulation of Fcp1-2 protein activity by centromeric DNA is discussed.