Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation
Migrasomes provide regional cues for organ morphogenesis during zebrafish gastrulation
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迁移体为斑马鱼原肠胚形成期间的器官形态发生提供区域线索
DOI：
10.1038/s41556-019-0358-6
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发表时间：
2019-08-01
影响因子：
21.3
通讯作者：
Yu, Li
中科院分区：
文献类型：
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作者：
Jiang, Dong;Jiang, Zheng;Yu, Li
移行小体是新近发现的一种囊泡细胞器，它形成于迁移细胞后面的收缩纤维上。目前尚不清楚体内是否存在迁移体，如果存在，则还不清楚其在生物体中的功能。在这里，我们证明了在斑马鱼原肠形成过程中形成了迁移体，信号分子，如趋化因子，在迁移体中得到了丰富。我们进一步证明了Tspan4和Tspan7是形成迁移体所必需的。斑马鱼MZtspan4a和MZtspan7突变体的器官形态发生受损。从机制上讲，迁移体富集在胚盾下的空腔上，在那里它们作为化学吸引剂，确保背侧前体细胞在植物上正确定位在胚盾附近，从而影响器官的形态发生。我们的研究表明，迁移小体是提供特定生化信息以协调器官形态发生的信号细胞器。
Migrasomes are recently identified vesicular organelles that form on retraction fibres behind migrating cells. Whether migrasomes are present in vivo and, if so, the function of migrasomes in living organisms is unknown. Here, we show that migrasomes are formed during zebrafish gastrulation and signalling molecules, such as chemokines, are enriched in migrasomes. We further demonstrate that Tspan4 and Tspan7 are required for migrasome formation. Organ morphogenesis is impaired in zebrafish MZtspan4a and MZtspan7 mutants. Mechanistically, migrasomes are enriched on a cavity underneath the embryonic shield where they serve as chemoattractants to ensure the correct positioning of dorsal forerunner cells vegetally next to the embryonic shield, thereby affecting organ morphogenesis. Our study shows that migrasomes are signalling organelles that provide specific biochemical information to coordinate organ morphogenesis.