Assembly of water-soluble AIE-active fluorescent organic nanoparticles for ratiometric detection of hypochlorite in living cells.
Assembly of water-soluble AIE-active fluorescent organic nanoparticles for ratiometric detection of hypochlorite in living cells.
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组装水溶性 AIE 活性荧光有机纳米粒子，用于活细胞中次氯酸盐的比例检测。
DOI：
10.1002/asia.202001325
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发表时间：
2020-12
影响因子：
4.1
通讯作者：
Guo‐wen Xing
中科院分区：
文献类型：
--
作者：
Yi‐chen Liu;Wei Du;Guang-jian Liu;Wei Zhou;Xiao‐jie Gao;Guo‐wen Xing
次氯酸（HOCl）在许多生理过程中起着至关重要的作用，并被广泛用作漂白剂，除臭剂和杀菌剂。基于HOCl引发的水解反应和聚集诱导发射（AIE）效应，设计了一种含有亲水性糖单元和疏水性四苯乙烯单元的两亲性腙类荧光分子THG-1。通过液相色谱-质谱联用实验和扫描电子显微镜（SEM）实验验证了检测机理。对比实验表明，乳糖单元和腙连接基的数目对THG-1的组装和HOCl的检测至关重要。此外，THG-1被成功地用于活细胞中外源性和内源性HOCl的成像。
Hypochlorous acid (HOCl) plays a crucial role in many physiological processes and is widely used as bleach, deodorant and fungicide. In this work, we designed an amphiphilic hydrazone fluorescent molecule THG-1 containing hydrophilic sugar units and hydrophobic tetraphenylethylene unit for ratiometric detection of HOCl with high sensitivity and excellent selectivity based on HOCl-triggered hydrolyzation reaction and aggregation-induced emission (AIE) effect. The detection mechanism was verified by liquid chromatograph mass spectrometry experiments and scanning electron microscope (SEM) tests. Contrast experiments revealed that the numbers of lactose unit and hydrazone linker were essential for assembly of THG-1 and detection of HOCl. In addition, THG-1 was successfully used for imaging of exogenous and endogenous HOCl in living cells.
DOI：
10.1021/j100511a013
发表时间：
1978-11
期刊：
The Journal of Physical Chemistry
影响因子：
--
作者：
L. Molina;M. Molina
通讯作者：
L. Molina;M. Molina
影响因子：
6.2
作者：
Claver, JB;Mirón, MCV;Capitán-Vallvey, LF
通讯作者：
Capitán-Vallvey, LF
影响因子：
6.1
作者：
L. Guerra;C. Pereira;P. Andrade;M. Rodrigues;F. Ferreres;P. G. de Pinho;R. Seabra;P. Valentão
通讯作者：
L. Guerra;C. Pereira;P. Andrade;M. Rodrigues;F. Ferreres;P. G. de Pinho;R. Seabra;P. Valentão