LOCALIZATION OF HEPARIN-BINDING EPIDERMAL GROWTH FACTOR-LIKE GROWTH-FACTOR IN HUMAN GASTRIC-MUCOSA
LOCALIZATION OF HEPARIN-BINDING EPIDERMAL GROWTH FACTOR-LIKE GROWTH-FACTOR IN HUMAN GASTRIC-MUCOSA
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DOI：
10.1016/0016-5085(95)90562-6
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发表时间：
1995-10-01
期刊：
影响因子：
29.4
通讯作者：
MATSUZAWA, Y
中科院分区：
文献类型：
--
作者：
MURAYAMA, Y;MIYAGAWA, JI;MATSUZAWA, Y
背景与目的：肝素结合表皮生长因子样生长因子(HB-EGF)是近年来发现的一种表皮生长因子家族成员。在某些类型的胃上皮细胞中已检测到可与HB-EGF结合的EGF受体。本研究的目的是探讨胃上皮细胞是否产生HB-EGF以维持正常的胃粘膜。方法：采用Northern杂交和免疫组织化学方法检测HB-EGF蛋白在人胃粘膜中的表达和产物，并对产生HB-EGF蛋白的细胞类型进行鉴定。结果：HB-EGF信使RNA存在于机体和胃窦中。免疫组织化学染色显示HB-EGF主要定位于胃底腺的壁细胞和幽门腺的胃泌素细胞。在壁细胞和胃泌素细胞中可见EGF受体的免疫反应阳性，在增殖区的表面上皮细胞和粘液颈细胞中有微弱的EGF受体免疫反应。结论：HB-EGF主要由壁细胞和胃泌素细胞合成，可能通过其表面EGF受体以自分泌和/或旁分泌的方式调节胃粘膜细胞的增殖和分化。
Background & Aims: Heparin-binding epidermal growth factor-like growth factor (HB-EGF) has been recently identified as a member of the EGF family. EGF receptors to which HB-EGF can bind have been detected in some types of gastric epithelial cells. The aim of this study was to investigate whether HB-EGF is produced in gastric epithelial cells to maintain normal gastric mucosa. Methods: Gene expression and production of HB-EGF protein were investigated using Northern hybridization and immunohistochemistry, and the types of cells producing this protein were determined in human gastric mucosa. Results: HB-EGF messenger RNA was detected in the body and antrum. Immunohistochemical staining showed that HB-EGF was localized mainly in parietal cells of fundic glands and in gastrin cells of pyloric glands. Also, the immunoreactivity of EGF receptors was observed in parietal cells and gastrin cells and faintly in surface epithelial cells and mucous neck cells of the proliferative zone. Conclusions: The results suggest that HB-EGF is synthesized mainly in parietal cells and gastrin cells and may act in an autocrine and/or paracrine manner in the regulation of proliferation and differentiation of the gastric mucosal cells through their surface EGF receptors.