MagiProbe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator.
MagiProbe: a novel fluorescence quenching-based oligonucleotide probe carrying a fluorophore and an intercalator.
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MagiProbe：一种新型的基于荧光猝灭的寡核苷酸探针，带有荧光团和嵌入剂。
DOI：
10.1093/nar/gnf096
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发表时间：
2002
影响因子：
14.9
通讯作者：
A. Yamane
中科院分区：
文献类型：
--
作者：
A. Yamane
荧光是分子诊断中最受欢迎的信号技术。基于荧光能量转移的方法是强大的均相分析工具。一种新的寡核苷酸探针，命名为MagiProbe，这是简单的使用，描述，和信息与目标形成的双链体。探针内部具有荧光团和嵌入剂。在没有靶序列的情况下，其荧光被嵌入剂淬灭。在与靶序列杂交时，由于嵌入猝灭的干扰，探针发射标记的荧光。此外，与单碱基错配靶杂交的MagiProbe比与完全匹配的靶杂交的MagiProbe发出更少的荧光。因此，它可以检测与靶的双链形式的单个碱基差异。
Fluorescence is the favored signaling technology for molecular diagnoses. Fluorescence energy transfer-based methods are powerful homogeneous assay tools. A novel oligonucleotide probe, named MagiProbe, which is simple to use, is described, and information given about the duplex formed with a target. The probe internally has a fluorophore and an intercalator. Its fluorescence is quenched by the intercalator in the absence of a target sequence. On hybridization with a target sequence, the probe emits marked fluorescence due to the interference in quenching by intercalation. Furthermore, MagiProbe hybridized with a single-base mismatched target emits less fluorescence than with a perfect matched target. It therefore can detect a single base difference in a double-stranded form with a target.
影响因子：
3.6
作者：
S. M. Morais;G. Wilkinson;J. Blaisdell;U. Meyer;K. Nakamura;J. Goldstein
通讯作者：
S. M. Morais;G. Wilkinson;J. Blaisdell;U. Meyer;K. Nakamura;J. Goldstein