Integrated solid-state nanopore platform for nanopore fabrication via dielectric breakdown, DNA-speed deceleration and noise reduction.
Integrated solid-state nanopore platform for nanopore fabrication via dielectric breakdown, DNA-speed deceleration and noise reduction.
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DOI：
10.1038/srep31324
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发表时间：
2016-08-08
影响因子：
4.6
通讯作者：
Takeda K
中科院分区：
文献类型：
--
作者：
Goto Y;Yanagi I;Matsui K;Yokoi T;Takeda K
固态纳米孔在DNA测序中的实际应用需要纳米孔的容易制造、DNA移动速度的降低和离子电流噪声的降低。在这里，我们报告了一个集成的纳米孔平台，具有纳米珠结构，可以减缓DNA的运动，绝缘聚酰亚胺层可以降低噪音。为了实现快速的纳米孔制造，我们在我们的系统中引入了受控介电击穿（CDB）过程。DNA易位实验显示，通过CDB工艺产生单个纳米孔，而不会牺牲在1 MHz下将DNA移动速度降低高达10 μs/碱基或将噪声降低高达600 pArms的性能。我们的平台为DNA测序过程中的下一步提供了必要的组件。
The practical use of solid-state nanopores for DNA sequencing requires easy fabrication of the nanopores, reduction of the DNA movement speed and reduction of the ionic current noise. Here, we report an integrated nanopore platform with a nanobead structure that decelerates DNA movement and an insulating polyimide layer that reduces noise. To enable rapid nanopore fabrication, we introduced a controlled dielectric breakdown (CDB) process into our system. DNA translocation experiments revealed that single nanopores were created by the CDB process without sacrificing performance in reducing DNA movement speed by up to 10 μs/base or reducing noise up to 600 pArms at 1 MHz. Our platform provides the essential components for proceeding to the next step in the process of DNA sequencing.