An integrin binding motif in TLN-1/talin plays a minor role in motility and ovulation.
An integrin binding motif in TLN-1/talin plays a minor role in motility and ovulation.
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DOI：
10.17912/micropub.biology.000726
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发表时间：
2023
影响因子：
--
通讯作者：
Cram, Erin J
中科院分区：
文献类型：
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作者：
Sadeghian, Fereshteh;Ibrahim, Ibrahim;Ravichandran, Lokesh;Henderson, Grace;Acharya, Anisha;Wang, Lianzijun;Lee, Myeongwoo;Cram, Erin J
TLN-1/talin 是一种保守的粘着斑蛋白，形成整合素细胞质尾部和肌动蛋白细胞骨架之间连接的一部分。在秀丽隐杆线虫中，TLN-1 在横纹肌和性腺鞘细胞中强烈表达。在这里，我们报告了 CRISPR 生成的 TLN-1 等位基因 TLN-1(W387A)，预计会影响 talin 与整合素的结合，导致轻微的表型，包括运动缺陷和排卵缺陷。野生型和 TLN-1(W387A) 动物的体壁肌肉、受精囊和鞘中肌动蛋白细胞骨架的排列似乎相同。该分析表明，TLN-1 中的 W387 对体内 talin 与整合素的结合没有重大影响。
TLN-1/talin is a conserved focal adhesion protein that forms part of the linkage between the cytoplasmic tail of integrin and the actin cytoskeleton. In C. elegans , TLN-1 is expressed strongly in striated muscle and the gonadal sheath cells. Here, we report that a CRISPR-generated TLN-1 allele TLN-1(W387A), predicted to affect binding of talin to integrins, results in mild phenotypes, including motility defects and ovulation defects. The arrangement of the actin cytoskeleton in the body wall muscles, spermatheca, and sheath appears identical in wild type and TLN-1(W387A) animals. This analysis suggests that W387 in TLN-1 does not have a major effect on the binding of talin to integrin in vivo .