Sex differences in auditory brainstem response audiograms from vasopressin-deficient Brattleboro and wild-type Long-Evans rats
Sex differences in auditory brainstem response audiograms from vasopressin-deficient Brattleboro and wild-type Long-Evans rats
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DOI：
10.1371/journal.pone.0222096
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发表时间：
2019-08-30
期刊：
影响因子：
3.7
通讯作者：
Dent, Micheal L.
中科院分区：
文献类型：
--
作者：
Charlton, Payton E.;Schatz, Kelcie C.;Dent, Micheal L.
老鼠是高度社会化的生物，在社会交往中产生超声波发声（USV）。Brattleboro大鼠，一种Long-Evans衍生的大鼠，由于Avp基因突变而缺乏加压素（AVP），表现出非典型的社会行为，包括在社会互动过程中具有改变的谱时特征的USV较少。目前还不清楚为什么Brattle-boro大鼠产生非典型USVs，但一个因素可能是它们与具有功能性加压素的野生型Long Evans大鼠之间的听觉敏锐度差异。先前的研究表明AVP水平的增加与听觉处理之间存在联系。此外，很少有研究探讨了Long-Evans大鼠听觉感知的性别差异。听觉灵敏度的性别差异已经在整个动物王国中被发现，但尚未在大鼠听力图中得到证实。本研究旨在测量雄性和雌性纯合型Brattleboro（Hom）、杂合型Brattleboro（Het）和野生型（WT）Long-Evans大鼠在1 - 64 kHz频率范围内的听性脑干反应（ABR）衍生听力图，以更好地了解AVP的作用和这些大鼠在听觉处理中的性别差异。我们未能检测到显着差异的ABR听力图的Hom，Het，和WT Long-Evans大鼠，表明不同水平的AVP不影响听觉处理。有趣的是，所有基因型的男性和女性的ABR阈值不同，男性表现出更高的阈值比女性。听觉灵敏度的性别差异在最低和最高频率，可能会影响USV的看法。这是第一次在大鼠听力图中发现性别差异。
Rats are highly social creatures that produce ultrasonic vocalizations (USVs) during social interactions. Brattleboro rats, a Long-Evans derived rat that lacks vasopressin (AVP) due to a mutation in the Avp gene, exhibit atypical social behavior, including fewer USVs with altered spectrotemporal characteristics during social interactions. It is unclear why Brattle-boro rats produce atypical USVs, but one factor could be differences in auditory acuity between them and wild-type Long Evans rats with functional vasopressin. Previous studies have suggested a link between increased levels of AVP and auditory processing. Additionally, few studies have investigated sex differences in auditory perception by Long-Evans rats. Sex differences in auditory acuity have been found throughout the animal kingdom, but have not yet been demonstrated in rat audiograms. This study aimed to measure auditory brainstem response (ABR) derived audiograms for frequencies ranging from 1 to 64 kHz in male and female homozygous Brattleboro (Hom), heterozygous Brattleboro (Het), and wild-type (WT) Long-Evans rats to better understand the role of AVP and sex differences in auditory processing by these rats. We failed to detect significant differences between the ABR audiograms of Hom, Het, and WT Long-Evans rats, suggesting that varying levels of AVP do not affect auditory processing. Interestingly, males and females of all genotypes did differ in their ABR thresholds, with males exhibiting higher thresholds than females. The sex differences in auditory acuity were significant at the lowest and highest frequencies, possibly affecting the perception of USVs. These are the first known sex differences in rat audiograms.