Comparison of Material-mediated Bone Regeneration Capacities of Sintered and Non-sintered Xenogeneic Bone Substitutes via 2D and 3D Data
Comparison of Material-mediated Bone Regeneration Capacities of Sintered and Non-sintered Xenogeneic Bone Substitutes via 2D and 3D Data
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DOI：
10.21873/invivo.11719
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发表时间：
2019-11-01
期刊：
影响因子：
2.3
通讯作者：
Rothamel, Daniel
中科院分区：
文献类型：
--
作者：
Kapogianni, Eleni;Barbeck, Mike;Rothamel, Daniel
背景/目的：本研究的目的是开发一种新的骨传导性指数，以确定骨替代材料（BSM）的骨愈合能力的基础上，三维微计算机断层扫描（mu-CT）数据。材料与方法：窦活检用于比较分析两种异种BSM（cerabone（R）和Bio-Oss（R））的整合行为。使用3D mu-CT和基于2D组织切片的组织形态测量数据集测量活检组织内的骨材料接触和组织分布。对两种BSM的组织反应进行显微镜分析。结果如下：骨传导性测量的3D和2D结果显示，两种BSM的材料-骨接触相当，但2D数据显著较低。在两组中测量组织分布时发现相同的结果。组织病理学分析显示两种BSM的组织反应相当。结论：骨传导指数是判断骨愈合能力的可靠指标。两种测量方法之间观察到的差异可归因于mu-CT数据的分辨率能力，其不允许BSM和骨组织之间的精确界面区分。基于组织学切片的组织形态计量学数据仍然可以进行更准确的评价。
Background/Aim: The aim of this study was the development of a new osteoconductivity index to determine the bone healing capacities of bone substitute materials (BSM) on the basis of 3D microcomputed tomographic (mu-CT) data. Materials and Methods: Sinus biopsies were used for the comparative analysis of the integration behavior of two xenogeneic BSM (cerabone (R) and Bio-Oss (R)). 3D mu-CT and data sets from histomorphometrical measurements based on 2D histological slices were used to measure the bone-material-contact and the tissue distribution within the biopsies. The tissue reactions to both BSM were microscopically analyzed. Results: The 3D and 2D results of the osteoconductivity measurements showed comparable material-bone contacts for both BSM, but the 2D data were significantly lower. The same results were found when tissue distribution was measured in both groups. The histopathological analysis showed comparative tissue reactions in both BSM. Conclusion: Osteoconductivity index is a reliable measurement parameter for determining the healing capacities of BSM. The observed differences between both measurement methods could be assigned to the resolution capacity of mu-CT data that did not allow for a precise interface distinction between both BSM and bone tissue. Histomorphometrical data based on histological slides still allow for a more exact evaluation.