Phenotypic, biochemical and genomic variability in generations of the rapeseed (Brassica napus L.) mutant lines obtained via chemical mutagenesis
Phenotypic, biochemical and genomic variability in generations of the rapeseed (Brassica napus L.) mutant lines obtained via chemical mutagenesis
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DOI：
10.1371/journal.pone.0221699
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发表时间：
2019-08-28
期刊：
影响因子：
3.7
通讯作者：
Muravenko, Olga, V
中科院分区：
文献类型：
--
作者：
Amosova, Alexandra, V;Zoshchuk, Svyatoslav A.;Muravenko, Olga, V
以双低油菜、“00”、B为材料，用0.2%甲基磺酸乙酯（EMS）诱变，研究了M2-M5代油菜的表型、生化和遗传变异。欧洲油菜维克罗斯EMS诱变诱导的突变体后代在形态和农艺性状上的广泛多样性导致了B的EMS群体的选择。napus和B. rapa-morphotypes。所获得的突变系种子具有高蛋白、低油、主要脂肪酸含量稳定等特点，可用于饲料生产。尽管EMS群体中各种减数分裂异常的水平有所增加，但核型分析和基于FISH的45 S和5S rDNA可视化表明，M2-M5植株的核型稳定性较高，因此，所获得的突变系可用于进一步的油菜改良。在B的几种植物的核型中发现了染色体结构重组。rapa型表明油菜化学诱变育种会导致突变后代细胞遗传学不稳定，因此，应包括核型检查。研究结果表明，低浓度EMS在油菜改良中具有很大的潜力。
The phenotypic, biochemical and genetic variability was studied in M2-M5 generations of ethyl methansulfonat (EMS, 0.2%) mutagenized rapeseed lines generated from canola, '00', B. napus cv. Vikros. EMS mutagenesis induced extensive diversity in morphological and agronomic traits among mutant progeny resulted in selection of EMS populations of B. napus- and B. rapa-morphotypes. The seeds of the obtained mutant lines were high-protein, low in oil and stabilized in contents of main fatty acids which make them useful for feed production. Despite the increased level of various meiotic abnormalities revealed in EMS populations, comparative karyotype analysis and FISH-based visualization of 45S and 5S rDNA indicated a high level of karyotypic stability in M2-M5 plants, and therefore, the obtained mutant lines could be useful in further rapeseed improvement. The revealed structural chromosomal reorganizations in karyotypes of several plants of B. rapa-type indicate that rapeseed breeding by chemical mutagenesis can result in cytogenetic instability in the mutant progeny, and therefore, it should include the karyotype examination. Our findings demonstrate that EMS at low concentrations has great potential in rapeseed improvement.