Cytolytic effector function is present in resting peripheral T lymphocytes.
Cytolytic effector function is present in resting peripheral T lymphocytes.
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静息外周 T 淋巴细胞中存在溶细胞效应功能。
DOI：
10.1093/intimm/4.11.1273
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发表时间：
1992
影响因子：
4.4
通讯作者：
Dupont,B
中科院分区：
文献类型：
--
作者：
Geisberg,M;Dupont,B
抗原特异性细胞毒素杀伤淋巴细胞（ctl）是免疫系统的主要效应功能之一。众所周知，作为TCR识别携带抗原的靶细胞的结果，静止的T淋巴细胞发育成完全活跃的抗原特异性ctl。相比之下，自然杀伤细胞（NK）在与适当的靶细胞接触后立即呈典型型。ctl和NK细胞的典型机制被认为包括其细胞质颗粒的内容，特别是成孔蛋白穿孔素。在这里，我们报告了静息外周CD3+CD8+T细胞的直接细胞溶解活性，这是TCR-CD3与靶细胞结合的结果；以小鼠OKT3杂交瘤（抗人CD3）为靶点。细胞毒性在含有记忆T细胞的CD8+CD45RO+细胞群中比在CD8+CD45RA+细胞群中更为明显；CD8+CD4−成熟胸腺细胞无细胞毒性。这些群体的细胞溶解潜能与穿孔素的存在与否有关。结果表明，T细胞的细胞溶解机制在胸腺后发展，可以立即被TCR-CD3刺激触发。
Antigen-specific cytotoxlc killer lymphocytes (CTLs) represent one of the major effector functions of the immune system. It is well established that, as a consequence of TCR recognition of the antigen-bearing target cell, resting T lymphocytes develop Into fully active antigen-specific CTLs. In contrast, natural killer (NK) cells are immediately tytic upon contact with an appropriate target cell. The tytic machinery of CTLs and NK cells Is thought to include the contents of their cytoplasmlc granules, in particular the pore-forming protein perforin. Here we report direct cytolytic activity by resting peripheral CD3+CD8+T cells as a result of TCR-CD3 binding to the target cell; the murine OKT3 hybridoma (anti-human CD3) was used as a target. The cytotoxicity was more pronounced In the CD8+CD45RO+population, which contains ‘memory’ T cells, than In the reciprocal CD8+CD45RA+subset; CD8+CD4−mature thymocytes were non-cytotoxlc. The cytolytic potential of these populations correlated with the presence or absence of perforin. The results demonstrate that the cytolytic machinery of T cells develops post-thymically and can be Immediately triggered by TCR-CD3 stimulation.