Mechanosensitive ion channels in cultured sensory neurons of neonatal rats
Mechanosensitive ion channels in cultured sensory neurons of neonatal rats
复制标题
DOI：
10.1523/jneurosci.22-04-01238.2002
复制
发表时间：
2002-02-15
影响因子：
5.3
通讯作者：
Oh, U
中科院分区：
文献类型：
--
作者：
Cho, HW;Shin, J;Oh, U
机械敏感离子通道存在于多种细胞中。然而，我们对引起感觉神经元机械敏感性的离子通道知之甚少。我们在培养的背根神经节神经元中测试的2962个膜斑块中的1390个中发现了两种最常见但不同类型的MS通道。两个质谱通道表现出不同的阈值，因此被称为低阈值（LT）和高阈值（HT）质谱通道，并且对压力敏感。这两个通道保留了不同的单通道电导和电流-电压关系：LT和HT通道分别具有向外整流和线性I-V关系的大通道和小通道电导。LT和HT质谱通道均可通过单价阳离子和Ca2+，并被质谱通道阻滞剂钆阻断。秋水仙碱和细胞松弛素D显著降低了两个MS通道的活性，表明细胞骨架元素支持机械敏感性。这两种类型的质谱通道主要存在于直径为
Mechanosensitive (MS) ion channels are present in a variety of cells. However, very little is known about the ion channels that account for mechanical sensitivity in sensory neurons. We identified the two most frequently encountered but distinct types of MS channels in 1390 of 2962 membrane patches tested in cultured dorsal root ganglion neurons. The two MS channels exhibited different thresholds, thus named as low-threshold (LT) and high-threshold (HT) MS channels, and sensitivity to pressure. The two channels retained different single-channel conductances and current-voltage relationships: LT and HT channels elicited large- and small-channel conductance with outwardly rectifying and linear I-V relationships, respectively. Both LT and HT MS channels were permeable to monovalent cations and Ca2+ and were blocked by gadolinium, a blocker of MS channels. Colchicine and cytochalasin D markedly reduced the activities of the two MS channels, indicating that cytoskeletal elements support the mechanosensitivity. Both types of MS channels were found primarily in small sensory neurons with diameters of