Involvement of hydrogen peroxide and nitric oxide in salt resistance in the calluses from Populus euphratica.
Involvement of hydrogen peroxide and nitric oxide in salt resistance in the calluses from Populus euphratica.
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DOI：
10.1111/j.1365-3040.2007.01667.x
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发表时间：
2007-07
期刊：
影响因子：
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通讯作者：
Feng Zhang;Yuping Wang;Yingli Yang;Hao Wu;Di Wang;Jianquan Liu
中科院分区：
文献类型：
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作者：
Feng Zhang;Yuping Wang;Yingli Yang;Hao Wu;Di Wang;Jianquan Liu
一氧化氮 (NO) 和过氧化氢 (H2O2) 在非生物和生物胁迫下作为植物中的信号分子。具有耐盐性的胡杨愈伤组织被用来研究 NO 和 H2O2 在植物适应耐盐性过程中的相互作用。在愈伤组织中鉴定出一氧化氮合酶 (NOS) 活性，并且该活性是在 150 mM NaCl 处理下诱导的。在150 mM NaCl处理下，胡杨愈伤组织中钠（Na）百分比下降，但钾（K）百分比和K/Na比值增加。葡萄糖/葡萄糖氧化酶（G/GO，H2O2 供体）和硝普钠（SNP，NO 供体）的应用表明，H2O2 和 NO 都会以浓度依赖性方式导致 K/Na 比增加。二亚苯基碘鎓（DPI，一种 NADPH 氧化酶抑制剂）通过增加 Na 百分比、降低 K 百分比和 K/Na 比来抵消 H2O2 和 NO 的影响。 NG-monomethyl-L-Arg monoacetate（NMMA，一种 NO 合酶抑制剂）和 2-苯基-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxyde（PTIO，一种特定的 NO 清除剂）只能逆转 NO 效应，但不能阻断 H2O2 效应。 DPI 和 NMMA 处理可逆转盐胁迫引起的质膜 (PM) H+ -ATP 酶活性增加。外源H2O2增加了PM H+ -ATP酶的活性，但NMMA和PTIO不能减弱这种作用。 NO 诱导的 PM H+ -ATPase 增加可以被 NMMA 和 PTIO 逆转，但不能被 DPI 逆转。 Western blot分析表明，NO和H2O2刺激胡杨愈伤组织中PM H+ -ATP酶的表达。这些结果表明，NO 和 H2O2 作为中间分子，通过增加 K/Na 比来诱导板条胁迫下胡杨愈伤组织的耐盐性，这取决于 PM H+ -ATPase 活性的增加。
Nitric oxide (NO) and hydrogen peroxide (H2O2) function as signalling molecules in plants under abiotic and biotic stresses. Calluses from Populus euphratica, which show salt tolerance, were used to study the interaction of NO and H2O2 in plant adaptation to salt resistance. The nitric oxide synthase (NOS) activity was identified in the calluses, and this activity was induced under 150 mM NaCl treatment. Under 150 mM NaCl treatment, the sodium (Na) percentage decreased, but the potassium (K) percentage and the K/Na ratio increased in P. euphratica calluses. Application of glucose/glucose oxidase (G/GO, a H2O2 donor) and sodium nitroprusside (SNP, a NO donor) revealed that both H2O2 and NO resulted in increased K/Na ratio in a concentration-dependent manner. Diphenylene iodonium (DPI, an NADPH oxidase inhibitor) counteracted H2O2 and NO effect by increasing the Na percentage, decreasing the K percentage and K/Na ratio. NG-monomethyl-L-Arg monoacetate (NMMA, an NO synthase inhibitor) and 2-phenyl-4,4,5,5-tetramethyl-imidazoline-1-oxyl-3-oxyde (PTIO, a specific NO scavenger) only reversed NO effect, but did not block H2O2 effect. The increased activity of plasma membrane (PM) H+ -ATPase caused by salt stress was reversed by treatment with DPI and NMMA. Exogenous H2O2 increased the activity of PM H+ -ATPase, but the effect could not be diminished by NMMA and PTIO. The NO-induced increase of PM H+ -ATPase can be reversed by NMMA and PTIO, but not by DPI. Western blot analysis demonstrated that NO and H2O2 stimulated the expression of PM H+ -ATPase in P. euphratica calluses. These results indicate that NO and H2O2 served as intermediate molecules in inducing salt resistance in the calluses from P. euphratica under slat stress by increasing the K/Na ratio, which was dependent on the increased PM H+ -ATPase activity.