Genetic mapping reveals BjRf as a candidate gene controlling fertility restoration of the oxa CMS in Brassica juncea
Genetic mapping reveals BjRf as a candidate gene controlling fertility restoration of the oxa CMS in Brassica juncea
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遗传图谱揭示 BjRf 作为控制芥菜 oxa CMS 育性恢复的候选基因
DOI：
10.1007/s00122-021-03767-5
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发表时间：
2021-06-26
影响因子：
5.4
通讯作者：
Wan, Zhengjie
中科院分区：
文献类型：
--
作者：
Cheng, Qiqi;Yao, Peijie;Wan, Zhengjie
关键信息利用图位克隆和BSA方法从芥菜型油菜A05染色体上的23-kb区域分离到一个雄性育性恢复候选基因BjRf。细胞质雄性不育/育性恢复（CMS/Rf）系统已广泛应用于植物杂种优势的培育。它也为研究细胞核协同进化和相互作用提供了宝贵的资源。oxa CMS是在芥菜型油菜（Brassica juncea，B. juncea），已广泛应用于该物种杂种优势的开发和利用。然而，oxa CMS育性恢复基因BjRf还未见报道。本研究通过连续测交和人工选择，成功地构建了一个稳定的恢复系。此外，在B的A05染色体上的23-kb区域定位了一个新的Rf基因。通过群体分离分析（BSA）和图位克隆相结合的方法，将芥菜型油菜（juncea）与中国芥菜型油菜（Juncea）进行了遗传距离分析，遗传距离为0.5cM。最后，BjuA017917，一个非PPR Rf基因编码的鸟苷核苷酸二磷酸解离抑制剂（GDI），被认为是候选基因的oxa CMS系的育性恢复在B。Juncea。此外，还开发了一个用于芥菜型油菜分子标记辅助选择的功能标记--
Key message A candidate gene for male fertility restoration in Brassica juncea, BjRf, was isolated from a 23-kb interval on chromosome A05 using map-based cloning and BSA methods. The cytoplasmic male sterility/fertility restoration (CMS/Rf) system has been extensively used for heterosis in plants. It also provides valuable resources for studying mitochondrial-nuclear coevolution and interaction. The oxa CMS, which is a new CMS type reported in Brassica juncea (B. juncea), has been broadly used in the exploitation and application of heterosis in this species. However, the oxa CMS fertility restorer gene BjRf has not been reported. In this study, a stable restorer line was successfully constructed via continuous testcross and artificial selection. Besides, a new Rf gene was mapped in a 23-kb region on chromosome A05 in B. juncea with a genetic distance of 0.5 cM by the method incorporating bulk segregant analysis (BSA) and conventional map-based cloning. Finally, BjuA017917, a non-PPR Rf gene encoding a guanosine nucleotide diphosphate dissociation inhibitor (GDI), is proposed to be the candidate gene for fertility restoration of the oxa CMS line in B. juncea. Moreover, a functional marker, CRY3, was developed for marker-assisted selection for Brassica juncea breeding.