Differential expression of bladder neurotrophic factor mRNA in male and female rats after bladder outflow obstruction.
Differential expression of bladder neurotrophic factor mRNA in male and female rats after bladder outflow obstruction.
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膀胱流出道梗阻后雄性和雌性大鼠膀胱神经营养因子 mRNA 的差异表达
DOI：
10.1016/s0022-5347(05)64244-9
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发表时间：
2002
期刊：
影响因子：
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通讯作者：
Vizzard,MargaretA
中科院分区：
文献类型：
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作者：
Zvara,Peter;KlimentJr,Jan;DeRoss,AnthonyL;Irwin,BrianH;Malley,SusanE;Plante,MarkK;Vizzard,MargaretA
目的建立大鼠膀胱流出道梗阻模型，比较膀胱流出道梗阻6周后不同性别大鼠膀胱神经营养因子mRNA的表达。女性15例，男性19例；对照组10例，男性10例，女性10例。术后6周行清醒膀胱测压。结果与对照组相比，膀胱出口梗阻大鼠平均膀胱容量增加3倍，雌性增加4.4倍，膀胱重量分别增加2.2倍和4.3倍。膀胱出口梗阻的雌性大鼠和雄性大鼠的充盈压力和阈值压力显著增加，非排尿膀胱收缩发生率分别为100%和80%。梗阻后，无论性别，膀胱脑源性神经营养因子基因的表达都增加了8倍。膀胱神经生长因子的表达在梗阻后仅在雌性大鼠中增加2倍。结论通过在膀胱连接处放置管腔减缩结扎术建立了该雄性大鼠膀胱出口梗阻模型。膀胱出口梗阻大鼠膀胱脑源性神经营养因子基因表达的显著增加和神经生长因子基因表达的差异提示，额外的神经营养因子可能参与了与膀胱出口梗阻相关的下尿路神经可塑性，且这种作用可能与性别有关。
PurposeWe validated a male rat model of bladder outflow obstruction and compared the expression of bladder neurotrophic factor mRNA in male and female rats 6 weeks after bladder outlet obstruction.Materials and MethodsWe examined the proximal urethra in male Wistar rats. Urethral lumen reducing ligatures were placed in 15 females and 19 males, while 10 male and 10 female controls underwent sham surgery. Awake cystometry was performed 6 weeks after surgery. Ribonuclease protection assay was used to measure changes in bladder neurotrophic factor mRNA expression in the 2 sexes.ResultsAverage bladder capacity in rats with bladder outlet obstruction increased 3-fold in males and 4.4-fold in females compared with controls, while bladder weight increased 2.2 and 4.3-fold, respectively. Filling and threshold pressure increased significantly and nonvoiding bladder contractions were recorded in 100% of female and 80% of male rats with bladder outlet obstruction. An 8-fold increase in bladder brain derived neurotrophic factor mRNA was noted in each sex after obstruction. A 2-fold increase in bladder nerve growth factor mRNA after obstruction was only observed in females.ConclusionsThis male rat model of bladder outlet obstruction was created by placing lumen reducing ligatures at the urethrovesical junction. The dramatic increase in bladder brain derived neurotrophic factor mRNA expression and differential expression of nerve growth factor mRNA in male and female rats with bladder outlet obstruction suggest that additional neurotrophic factors may contribute to the lower urinary tract neuroplasticity associated with bladder outlet obstruction and this contribution may be gender dependent.