Crystallization and preliminary X-ray crystallographic studies of response regulator for cyanobacterial phytochrome, Rcp1.
Crystallization and preliminary X-ray crystallographic studies of response regulator for cyanobacterial phytochrome, Rcp1.
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蓝藻光敏色素 Rcp1 响应调节剂的结晶和初步 X 射线晶体学研究。
DOI：
10.1107/s0907444900009951
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发表时间：
2000
期刊：
影响因子：
--
通讯作者：
Eom,SH
中科院分区：
文献类型：
--
作者：
Im,YJ;Park,CM;Kim,JI;Yang,SS;Kang,JG;Rho,SH;Kim,JI;Song,WK;Song,PS;Eom,SH
的关键响应调节基因的光调节，rcp 1，从集胞藻属已过表达，纯化，随后结晶使用硫酸铵作为沉淀剂的形式适合于X-射线晶体学研究。在低温下收集的原始数据集分辨率为2.5μ m。晶体属六方晶系，空间群P63，晶胞参数a = B = 89.04（5），c = 60.29（3）nm。 的马修斯参数表明，Rcp 1结晶与每个不对称单元的两个分子。
The key response-regulator gene of light regulation, rcp1, from Synechocystis sp. has been overexpressed, purified and subsequently crystallized using ammonium sulfate as a precipitant in forms suitable for X-ray crystallographic studies. A native data set was collected to a resolution of 2.5 Å at cryogenic temperature. The crystals belong to the hexagonal space group P63, with unit-cell parameters a = b = 89.04 (5), c = 60.29 (3) Å. The Matthews parameter suggests that Rcp1 crystallizes with two molecules per asymmetric unit.