Quantification of single human dermal fibroblast contraction
Quantification of single human dermal fibroblast contraction
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DOI：
10.1089/ten.1998.4.281
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发表时间：
1998-09-01
影响因子：
--
通讯作者：
Sparrow, JC
中科院分区：
文献类型：
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作者：
Fray, TR;Molloy, JE;Sparrow, JC
通过视频显微镜和图像分析，定量分析了在可变形硅胶基质上培养的单个人真皮成纤维细胞（HDF）产生的收缩力。细胞收缩导致基底变形，表现为一系列垂直于细胞长轴的表面皱纹。局部表面变形是通过嵌入HDF粘附的表层的小乳胶珠的二维位移来测量的。使用校准过的玻璃微针来测量将表面拉伸已知量所需的力（表面刚度）。(1)根据乳胶珠的运动，计算细胞的收缩力。(2)在体内，这种力被认为会引起真皮层的收缩，从而促进伤口愈合。正常收缩对防止感染和水分流失至关重要。然而，异常的细胞行为被认为是造成各种伤口病理的原因，如肥厚性和瘢痕疙瘩。我们发现产生了2.65 mu N/cell的收缩力。这与单个平滑肌细胞产生的力相似(3)，比角化细胞测量的力大大约10倍，比先前发表的在胶原凝胶中培养的成纤维细胞的力大3个数量级。(4)我们的目标是了解决定极性和最大收缩力的机制，并研究HDF和肌成纤维细胞行为的差异。
Contraction forces produced by single, human dermal fibroblasts (HDF), cultured on deformable silicone substrata, were quantified using video microscopy and image analysis. Cell contraction causes deformation of the substrate, which appears as a series of surface wrinkles perpendicular to the long axis of the cell. Local surface deformation was measured from the two-dimensional displacement of small latex beads embedded in the surface layer to which the HDF adhere. A calibrated glass microneedle was used to measure the force required to stretch the surface by a known amount (the surface stiffness).(1) From the motion of the latex beads, the contractile forces of the cells were calculated.(2) In vivo, such forces are thought to cause contraction of the dermis and hence promote wound closure. Normal contraction is vital to prevent infection and water loss. However, aberrant cellular behaviour is thought to be responsible for a variety of wound pathologies, such as hypertrophic and keloid scarring. We have found that contractile forces of 2.65 mu N/cell were produced. This is similar to those produced by single smooth muscle cells(3) and approximately 10 times greater than the forces measured for keratocytes and three orders of magnitude greater than previously published values for fibroblasts that had been cultured in a collagen gel.(4) Our goal is to understand the mechanisms that determine the polarity and maximum force of contraction and also to study differences in the behavior of HDF and myofibroblasts.