DETERMINATION OF ISOPRENE IN HUMAN BREATH BY THERMAL-DESORPTION GAS-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION
DETERMINATION OF ISOPRENE IN HUMAN BREATH BY THERMAL-DESORPTION GAS-CHROMATOGRAPHY WITH ULTRAVIOLET DETECTION
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DOI：
10.1016/0378-4347(95)00207-y
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发表时间：
1995-10-06
影响因子：
3
通讯作者：
TAGESSON, C
中科院分区：
文献类型：
--
作者：
JONES, AW;LAGESSON, V;TAGESSON, C
本文介绍了一种分析人体呼气中异戊二烯(2-甲基-1，3-丁二烯)的新的、高灵敏度和专属性的方法。通过固体吸附材料吸入已知体积的呼出空气(150英里)以捕获痕量有机物质，然后在200℃下热解吸，然后用二极管阵列紫外检测-气相色谱测定异戊二烯。在较宽的呼气异戊二烯浓度范围内(0-12nmol/L)，线性关系良好(r=0.99)，低至0.10nmol/L的浓度很容易测量。16例健康受试者(男6例，女10例)均为非吸烟者，呼气异戊二烯浓度的平均值为3.73，中位数为3.36，散布浓度为1.60~10.33nmol/L。呼气异戊二烯浓度在性别之间没有统计学上的显著差异。平均值(+/-S.D.)同一受试者连续9次呼气异戊二烯浓度为3.69+/-0.60nmol/L，变异系数为16.3%。呼出的异戊二烯在白天和重复测试同一受试者的几天之间的变化要大得多。利用这种新的分析方法，可以高选择性和高灵敏度地研究异戊二烯在人体呼气中的排泄规律。
We describe a new, highly sensitive and specific method for the analysis of isoprene (2-methyl-1,3-butadiene) in human breath. A known volume of expired air (150 mi) was drawn through a solid sorbent material to capture trace organic substances, followed by thermal desorption at 200 degrees C and subsequent determination of isoprene by gas chromatography with diode-array ultraviolet detection. The calibration plot was linear (r = 0.99) over a wide range of breath isoprene concentrations (0-12 nmol/l), and levels down to 0.10 nmol/l were easily measurable. In sixteen healthy subjects (six men and ten women), all of whom were non-smokers, the mean, median and spread of breath isoprene concentrations were 3.73, 3.36 and 1.60-10.33 nmol/l, respectively. No statistically significant differences in the concentrations of breath isoprene were observed between the sexes. The mean ( +/- S.D.) concentration of breath isoprene in nine consecutive tests with the same subject was 3.69 +/- 0.60 nmol/l, and the coefficient of variation was 16.3%. Much larger variations in exhaled isoprene were seen during the day and also between days when the same subject was tested repeatedly. The excretion patterns of isoprene in human breath can be investigated with high selectivity and sensitivity with this new analytical method.