Gonadal Transcriptome Analysis of Pacific Abalone Haliotis discus discus: Identification of Genes Involved in Germ Cell Development
Gonadal Transcriptome Analysis of Pacific Abalone Haliotis discus discus: Identification of Genes Involved in Germ Cell Development
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DOI：
10.1007/s10126-018-9809-5
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发表时间：
2018-04
影响因子：
3
通讯作者：
Lingyun Yu;Dongdong Xu;H. Ye;H. Yue;S. Ooka;H. Kondo;R. Yazawa;Y. Takeuchi
中科院分区：
文献类型：
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作者：
Lingyun Yu;Dongdong Xu;H. Ye;H. Yue;S. Ooka;H. Kondo;R. Yazawa;Y. Takeuchi
鲍鱼性腺发育的分子机制目前还知之甚少。在这里，我们进行了太平洋鲍鱼的转录组分析，在大脑，卵巢，睾丸和未受精卵中发现基因。在已注释的unigenes中，48.6%的unigenes通过Venn图分析被鉴定为具有普遍或组织特异性表达。23个基因与性腺偏向的基因本体论（GO）条款首次获得。其次，通过筛选已知的与生殖细胞发育相关的基因名称，发现了36个基因。最后，通过使用与生殖相关的关键词查询合子表达的性腺基因（卵巢和睾丸）和母系表达的性腺基因（卵巢、睾丸和未受精卵）的注释的unigene数据库，获得17个基因。为了进一步验证这些基因的组织分布模式和亚细胞定位，RT-PCR和原位杂交进行使用unigene编码的生殖细胞标记，vasa，作为对照。结果表明，vasa主要在两性生殖细胞发育早期表达。其中一个候选基因，卵黄囊膜透明结构域蛋白12（ZP 12），在未成熟性腺的原始生殖细胞和成年雌性生殖细胞的早期发育阶段表达。本研究结果提示vasa和ZP 12参与了太平洋鲍生殖细胞的发育，ZP 12是一种特别有用的生殖细胞特异性标记。目前的性腺转录谱是一个广泛的资源，为未来的生殖分子生物学研究这一物种。
Little is known about the molecular mechanisms governing gonadal developmental processes in abalones. Here, we conducted transcriptome analysis of Pacific abaloneHaliotis discus discusfor gene discovery in the brain, ovary, testis, and unfertilized eggs. Among the annotated unigenes, 48.6% of unigenes were identified by Venn diagram analysis as having universal or tissue-specific expression. Twenty-three genes with gonad-biased gene ontology (GO) terms were first obtained. Secondly, 36 genes were found by screening known gene names related to germ cell development. Finally, 17 genes were obtained by querying the annotated unigene database for zygotically expressed gonadal genes (ovary and testis) and maternally expressed gonadal genes (ovary, testis, and unfertilized eggs) using keywords related to reproduction. To further verify tissue distribution pattern and subcellular localization of these genes, RT-PCR and in situ hybridization were performed using a unigene encoding a germ cell marker,vasa, as control. The results showed thatvasawas expressed mainly in the early developmental stages of germ cells in both sexes. One of the candidate genes,vitelline envelope zona pellucida domain protein 12(ZP12), was expressed in the primordial germ cells of immature gonad and early developmental stages of germ cells of the adult female. The results obtained from the present study suggest thatvasaandZP12are involved in germ cell development of Pacific abalone and thatZP12is an especially useful germ cell-specific marker in immature adults. The current gonadal transcriptome profile is an extensive resource for future reproductive molecular biology studies of this species.