Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging.
Evaluation of fluorophores for optimal performance in localization-based super-resolution imaging.
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DOI：
10.1038/nmeth.1768
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发表时间：
2011-11-06
期刊：
影响因子：
48
通讯作者：
Zhuang, Xiaowei
中科院分区：
文献类型：
--
作者：
Dempsey, Graham T.;Vaughan, Joshua C.;Chen, Kok Hao;Bates, Mark;Zhuang, Xiaowei
超分辨率荧光成像的一种方法使用顺序激活和单个荧光团的定位来实现高空间分辨率。在这里，它们对超分辨率图像质量的影响仅在这里进行了数量，我们对26个有机染料的开关特性进行了特征，这些特性将这些特性与超分辨率图像的质量联系起来范围，我们证明了低串扰，四色超分辨率成像。
One approach to super-resolution fluorescence imaging uses sequential activation and localization of individual fluorophores to achieve high spatial resolution. Essential to this technique is the choice of fluorescent probes — the properties of the probes, including photons per switching event, on/off duty cycle, photostability, and number of switching cycles, largely dictate the quality of super-resolution images. While many probes have been reported, a systematic characterization of the properties of these probes and their impact on super-resolution image quality has been described in only a few cases. Here, we quantitatively characterized the switching properties of 26 organic dyes and directly related these properties to the quality of super-resolution images. This analysis provides a set of guidelines for characterization of super-resolution probes and a resource for selecting probes based on performance. Our evaluation identified several photoswitchable dyes with good to excellent performance in four independent spectral ranges, with which we demonstrated low crosstalk, four-color super-resolution imaging.
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DOI：
10.1007/978-0-387-76497-9_4
发表时间：
2009-01-01
期刊：
HANDBOOK OF SINGLE-MOLECULE BIOPHYSICS
影响因子：
--
作者：
Dempsey, Graham T.;Wang, Wenqin;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
影响因子：
16.2
作者：
Dani, Adish;Huang, Bo;Bergan, Joseph;Dulac, Catherine;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
影响因子：
2.9
作者：
Flors, Cristina;Ravarani, Charles N. J.;Dryden, David. T. F.
通讯作者：
Dryden, David. T. F.
影响因子：
56.9
作者：
Bates, Mark;Huang, Bo;Zhuang, Xiaowei
通讯作者：
Zhuang, Xiaowei
影响因子：
16.6
作者：
Belov, Vladimir N.;Wurm, Christian A.;Hell, Stefan W.
通讯作者：
Hell, Stefan W.