Perioperative Elevation in Cell-Free DNA Levels in Patients Undergoing Cardiac Surgery: Possible Contribution of Neutrophil Extracellular Traps to Perioperative Renal Dysfunction.
Perioperative Elevation in Cell-Free DNA Levels in Patients Undergoing Cardiac Surgery: Possible Contribution of Neutrophil Extracellular Traps to Perioperative Renal Dysfunction.
复制标题
心脏手术患者无细胞DNA水平的围手术期升高：中性粒细胞外陷阱对围手术期肾功能障碍的可能贡献。
DOI：
10.1155/2016/2794364
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发表时间：
2016
影响因子：
1.4
通讯作者：
Makita K
中科院分区：
文献类型：
--
作者：
Qi Y;Uchida T;Yamamoto M;Yamamoto Y;Kido K;Ito H;Ohno N;Asahara M;Yamada Y;Yamaguchi O;Mitaka C;Tomita M;Makita K
背景本研究的目的是确定围手术期血清双链DNA（dsDNA）的变化，作为一个标志物可能反映中性粒细胞胞外陷阱浓度的样本接受心脏手术，并分析血清dsDNA浓度和围手术期肾功能不全之间的关系。方法.测量在先前进行的前瞻性多中心观察性研究期间收集的样品中的血清dsDNA浓度。80例择期心脏手术患者进行了研究。在基线、手术后即刻和手术后第二天（POD-1）采集血清样本。结果血清dsDNA浓度从基线（中位数，398 ng/mL [四分位数间距，372-475 ng/mL]）到手术后即刻（中位数，540 ng/mL [437-682 ng/mL]，p < 0.001）显著增加，POD-1降低了血清dsDNA浓度（中位数，323 ng/mL [256-436 ng/mL]）。POD-1时dsDNA浓度与血清肌酐浓度呈显著正相关（rs = 0.61，p < 0.001）。结论.在接受心脏手术的患者中，术后血清dsDNA浓度升高。dsDNA浓度的长期升高与围手术期肾功能不全相关。需要进一步的大规模研究来确定循环dsDNA的血清浓度和围手术期肾功能不全之间的关系。
Background. This study aimed to determine the perioperative change in serum double-strand DNA (dsDNA) as a marker potentially reflecting neutrophil extracellular trap concentration in samples from patients undergoing cardiac surgery and to analyze a relationship between serum dsDNA concentrations and perioperative renal dysfunction. Methods. Serum dsDNA concentrations in samples that were collected during a previously conducted, prospective, multicenter, observational study were measured. Eighty patients undergoing elective cardiac surgery were studied. Serum samples were collected at baseline, immediately after surgery, and the day after surgery (POD-1). Results. Serum dsDNA concentration was significantly increased from baseline (median, 398 ng/mL [interquartile range, 372–475 ng/mL]) to immediately after surgery (median, 540 ng/mL [437–682 ng/mL], p < 0.001), and they were reduced by POD-1 (median, 323 ng/mL [256–436 ng/mL]). The difference in serum creatinine concentration between baseline and POD-1 was correlated with dsDNA concentration on POD-1 (r s = 0.61, p < 0.001). Conclusions. In patients undergoing cardiac surgery, serum dsDNA concentration is elevated postoperatively. Prolonged elevation in dsDNA concentration is correlated with perioperative renal dysfunction. Further large-scale studies are needed to determine the relationship between serum concentration of circulating dsDNA and perioperative renal dysfunction.
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影响因子：
4
作者：
Malíčková K;Duricová D;Bortlík M;Hrušková Z;Svobodová B;Machková N;Komárek V;Fučíková T;Janatková I;Zima T;Lukáš M
通讯作者：
Lukáš M
影响因子：
2.8
作者：
Loegters, Tim;Paunel-Goerguelue, Adnana;Jaeger, Marcus
通讯作者：
Jaeger, Marcus
严重败血症患者的预后效用和无细胞DNA的表征。
DOI：
10.1186/cc11466
发表时间：
2012-08-13
期刊：
Critical care (London, England)
影响因子：
--
作者：
Dwivedi DJ;Toltl LJ;Swystun LL;Pogue J;Liaw KL;Weitz JI;Cook DJ;Fox-Robichaud AE;Liaw PC;Canadian Critical Care Translational Biology Group
通讯作者：
Canadian Critical Care Translational Biology Group
影响因子：
3.1
作者：
Margraf, Stefan;Loegters, Tim;Windolf, Joachim
通讯作者：
Windolf, Joachim
影响因子：
3.7
作者：
Uchida T;Ohno N;Asahara M;Yamada Y;Yamaguchi O;Tomita M;Makita K
通讯作者：
Makita K