Splitting hares and tortoises: a classification of neuronal immediate early gene transcription based on poised RNA polymerase II.
Splitting hares and tortoises: a classification of neuronal immediate early gene transcription based on poised RNA polymerase II.
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分裂野兔和乌龟：基于平衡 RNA 聚合酶 II 的神经元立即早期基因转录的分类。
DOI：
10.1016/j.neuroscience.2013.04.064
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发表时间：
2013
期刊：
影响因子：
3.3
通讯作者：
Dudek,SM
中科院分区：
文献类型：
--
作者：
Saha,RN;Dudek,SM
即时早期转录是神经元对环境刺激的反应的组成部分，并且服务于许多脑过程，包括发育、学习、程序性细胞死亡的触发以及对损伤和药物的反应。在刺激后，神经元在短时间内表达选定的几个基因，而不进行从头蛋白质翻译。被称为“遗传反应的门户”，这些立即早期基因（IEG）要么在刺激的几分钟内表达，要么在一小时内表达。在快速表达的神经元IEGs中，响应于神经元活性的生产性延伸通过组成型转录起始连同在启动子附近停滞的RNA聚合酶II而跳跃启动。在一小时后表达的IEG不依赖于这种机制。在此聚合酶II平衡的基础上，我们提出，立即早期基因可以分为两个不同的类：快速和延迟的IEG。这些类在神经元中可能的生物相关性进行了讨论。
Immediate early transcription is an integral part of the neuronal response to environmental stimulation and serves many brain processes including development, learning, triggers of programmed cell death, and reaction to injury and drugs. Following a stimulus, neurons express a select few genes within a short period of time without undergoing de novo protein translation. Referred to as the ‘gateway to genetic response’, these immediate early genes (IEGs) are either expressed within a few minutes of stimulation or later within the hour. In neuronal IEGs that are expressed rapidly, productive elongation in response to neuronal activity is jump-started by constitutive transcription initiation together with RNA polymerase II stalling in the vicinity of the promoter. IEGs expressed later in the hour do not depend on this mechanism. On the basis of this Polymerase II poising, we propose that the immediate early genes can be grouped in two distinct classes: the rapid and the delayed IEGs. The possible biological relevance of these classes in neurons is discussed.