Regional and segmental differences in the embryonic expression of a putative leech Hox gene, Lox2, by central neurons immunoreactive to FMRFamide-like neuropeptides
Regional and segmental differences in the embryonic expression of a putative leech Hox gene, Lox2, by central neurons immunoreactive to FMRFamide-like neuropeptides
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DOI：
10.1007/s10158-013-0161-1
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发表时间：
2014-03-01
影响因子：
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通讯作者：
Aisemberg, Gabriel O.
中科院分区：
文献类型：
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作者：
Gharbaran, Rajendra;Alvarado, Susana;Aisemberg, Gabriel O.
我们使用大鼠多克隆抗体对甲醛固定的整装胚胎进行免疫荧光实验，以表征胚胎发育过程中推定的水蛭Hox基因Lox 2的表达。主要目的是确定FMRFamide样免疫反应（FLI）神经元亚群的分化是否与Lox 2的表达域一致。Lox 2的最早表达在胚胎第4天的后部区域中相对较大的突出核中检测到，这是一个非常早期的阶段。Lox 2表达还在位于中间体神经节6（M6）-M21中的中枢神经元（位于CNS中的神经元）的子集中检测到。此外，Lox 2表达的一些片段特异性和片段重复的中央FLI神经元。Lox 2阳性FLI神经元包括一些以前确定的：最腹侧（RMV）神经元，腹侧（CV）神经元，和细胞261。成对的RMV位于所有的中间体神经节中，仅在M7-M19中表达Lox 2。CV神经元是支配体壁的圆形腹侧肌肉的特化运动神经元，在M7-M19中表达Lox 2。推定细胞261在M7-M12中表达Lox 2，其中Lox 1也表达。在其他节段神经节中未检测到细胞261的推定节段同源物中的FMRFamide染色。我们的研究结果表明Lox 2在非常早期的胚胎发育（CNS形成之前）中的作用，以及在分段重复和区域特异性FLI神经元的分化中的作用。
We performed immunofluorescence experiments using a rat polyclonal antibody on formaldehyde-fixed whole-mount embryos to characterize the expression of a putative leech Hox gene, Lox2, during embryonic development. The main goal was to determine whether the differentiation of subsets of FMRFamide-like immunoreactive (FLI) neurons coincide with the expression domain of Lox2. The earliest expression of Lox2 was detected in relatively large, prominent nuclei in the posterior region at embryonic day 4, a very early stage. Lox2 expression was also detected in subsets of central neurons (neurons located in the CNS) located in midbody ganglia 6 (M6)-M21. In addition, Lox2 was expressed by a number of segment-specific and segmentally repeated central FLI neurons. Lox2-positive FLI neurons of interest included some of those previously identified: the rostral most ventral (RMV) neurons, the circular ventral (CV) neurons, and cell 261. The paired RMVs, which are located in all midbody ganglia, expressed Lox2 only in M7-M19. The CV neurons, specialized motor neurons that innervate the circular ventral muscles of the body wall, expressed Lox2 in M7-M19. The putative cell 261 expressed Lox2 in M7-M12, where Lox1 is also expressed. FMRFamide staining in putative segmental homologs of cell 261 was not detected in other segmental ganglia. Our results suggest a role for Lox2 in very early embryonic development (before the formation of the CNS), and in the differentiation of segmentally repeated and region-specific FLI neurons.