A Protein Phosphatase 1 involved in correct nucleus positioning in trypanosomes
A Protein Phosphatase 1 involved in correct nucleus positioning in trypanosomes
复制标题
DOI：
10.1016/j.molbiopara.2013.11.001
复制
发表时间：
2013-11-01
影响因子：
1.5
通讯作者：
Kohl, Linda
中科院分区：
文献类型：
--
作者：
Gallet, Cecile;Demonchy, Raphael;Kohl, Linda
可逆的蛋白质磷酸化是细胞内功能的关键调节因素。在非洲锥虫（布氏锥虫）中，丝氨酸 - 苏氨酸磷酸酶PP1 - 3定位于细胞质。RNA干扰介导的PP1 - 3基因敲低导致前循环锥虫中细胞器和细胞区室的协同重排。这些寄生虫的细胞核位于细胞的后端。动基体非常靠近细胞核，且通常位于更靠前的位置。在正常的前循环细胞中位于细胞核和动基体之间的溶酶体区室，现在移向细胞的前端。对于胞质分裂至关重要的鞭毛附着区仍然存在，能够启动分裂沟和细胞分裂。这些适应性变化使得分裂的细胞能够在子细胞之间分配其细胞器，并正常增殖。因此，PP1 - 3对于维持前循环锥虫细胞的细胞内组织至关重要。（c）2013爱思唯尔公司。版权所有。
Reversible protein phosphorylation is a key regulator in intracellular functions. In the African trypanosome, Trypanosoma brucei, the serine-threonine phosphatase PP1-3, is localised in the cytoplasm. RNAi mediated knockdown of PP1-3 leads to a coordinated rearrangement of cellular organelles and compartments in the procyclic trypanosome. These parasites display their nucleus at the very posterior end of the cell. The kinetoplast is very close to the nucleus, and often located in a more anterior position. The lysosomal compartment, which in a normal procyclic cell is situated between nucleus and kinetoplast, is now positioned towards the anterior end of the cell. The Flagellum Attachment Zone, essential for cytokinesis, is still constructed, allowing initiation of the cleavage furrow and cell division. These adaptations allow dividing cells to distribute their organelles among the daughter cells and to proliferate normally. PP1-3 is therefore essential in conserving the intracellular organisation of the procyclic trypanosome cell. (c) 2013 Elsevier B.V. All rights reserved.